Three silver(I) dipeptide complexes [Ag(GlyGly)]n(NO3)n (AgGlyGly), [Ag2(GlyAla)(NO3)2]n (AgGlyAla) and [Ag2(HGlyAsp)(NO3)]n (AgGlyAsp) were prepared, investigated and characterized by vibrational spectroscopy (mid-IR), elemental and thermogravimetric analysis and mass spectrometry. For AgGlyGly, X-ray crystallography was also performed. Their stability in biological testing media was verified by time-dependent NMR measurements. Their in vitro antimicrobial activity was evaluated against selected pathogenic microorganisms. Moreover, the influence of silver(I) dipeptide complexes on microbial film formation was described. Further, the cytotoxicity of the complexes against selected cancer cells (BLM, MDA-MB-231, HeLa, HCT116, MCF-7 and Jurkat) and fibroblasts (BJ-5ta) using a colorimetric MTS assay was tested, and the selectivity index (SI) was identified. The mechanism of action of Ag(I) dipeptide complexes was elucidated and discussed by the study in terms of their binding affinity toward the CT DNA, the ability to cleave the DNA and the ability to influence numbers of cells within each cell cycle phase. The new silver(I) dipeptide complexes are able to bind into DNA by noncovalent interaction, and the topoisomerase I inhibition study showed that the studied complexes inhibit its activity at a concentration of 15 μM.

• Klíčová slova
• DNA interaction, anticancer activity, antimicrobial activity, cell cycle arrest, crystal structure, dipeptide, silver(I) complexes, stability, topoisomerase I inhibition,
• MeSH
• antiinfekční látky chemická syntéza   chemie   farmakologie   MeSH
• buněčný cyklus účinky léků   MeSH
• chemické jevy MeSH
• dipeptidy chemie   MeSH
• komplexní sloučeniny chemická syntéza   chemie   farmakologie   MeSH
• krystalografie rentgenová MeSH
• lidé MeSH
• molekulární konformace MeSH
• nádorové buněčné linie MeSH
• protinádorové látky chemická syntéza   chemie   farmakologie   MeSH
• simulace molekulární dynamiky MeSH
• spektrální analýza MeSH
• stabilita léku MeSH
• stříbro chemie   MeSH
• techniky syntetické chemie MeSH
• termogravimetrie MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiinfekční látky MeSH
• dipeptidy MeSH
• komplexní sloučeniny MeSH
• protinádorové látky MeSH
• stříbro MeSH

Two silver(i) complexes, {[Ag7(2-pypo)3(NO3)]} n (1) and [Ag(3-pypoH)(3-pypoH2)] (2) (pypoH2 - pyridylphosphonic acid) were prepared and characterized by relevant methods in the solid state (elemental, spectral, thermal and structural analysis). Typical argentophilic interactions were observed in complex 1. The synthesized Ag(i) complexes were tested toward reduction to silver(0) using short wavelength UV-radiation and they revealed remarkable light-insensitivity in comparison to silver nitrate. SEM experiments were performed to observe the reduction of silver after the UV-light exposure of the samples. Light-insensitivity with the examined thermal stability and insolubility of the new complexes demonstrates their high potential to be used as light-insensitive materials in medical devices.

• Publikační typ
• časopisecké články MeSH