Small non-coding RNAs control normal development and differentiation in the embryo. These regulatory molecules play a key role in the development of human diseases and are used often today for researching new treatments for different pathologies. In this study, CaCo2 colorectal adenocarcinoma cells were initially epigenetically reprogrammed and transformed into CD4+ cells with nano-sized complexes of amphiphilic poly-(N-vinylpyrrolidone) (PVP) with miRNA-152 and piRNA-30074. The transformation of cells was confirmed by morphological and genetic changes in the dynamic of reprogramming. CD4+ lymphocytes marker was detected using immunofluorescence. Amphiphilic poly-(N-vinylpyrrolidone)/small non-coding RNAs complexes were investigated for transfection efficiency and duration of transfection of CaCo2 colorectal adenocarcinoma cells using fluorescence.

• Klíčová slova
• AGO2, argonaute 2, Amphiphilic poly-(N-vinylpyrrolidone), BACH1, BTB domain and CNC homolog 1, CD, cluster of differentiation, CaCo2 colorectal adenocarcinoma, DICER1, ribonuclease III, DNMT1, DNA methyltransferase 1, DTT, dithyothreitol, ERK1/2, extracellular signal regulated kinase ½, FGF2, fibroblast growth factor 2, GITR3A, glucocorticoid-induced TNFR-related protein, H3K9me3, tri-methyl lysine 9 of histone H3, HILI, human piwi, HMOX1, heme oxygenase 1, HOXA10, homebox A10, ICOS1B, inducible T-cell co-stimulator, IL, interleukin, KIR1DL2, CD158b, expressed on natural killer cells and a subset of T cells, MKI-67, marker of proliferation ki-67, OCT4, octamer-binding transcription factor 4, PIWIL1, piwi-like protein 1, PNVP, poly-(N-vinylpyrrolidone), Polymer carriers, RB1, retinoblastoma 1, Reprogramming, SncRNAs, small non-coding RNAs, TE, transposon elements, TGFBR2, transforming growth factor beta receptor 2, TNFRS6B, TNF receptor superfamily 6B, TSS, transcriptional start sites, VMAF, musculoaponeurotic fibrosarcoma, Wnt-1, wingless type MMTV integration site family, member 1, iPS, induced pluripotent stem cells, mTOR, mechanistic target of rapamycin, miR, micro-RNA, miRNA-152, piR, piwi-interacting RNA, P-element induced wimpy testis interacting RNA, piRNA-30074,
• Publikační typ
• časopisecké články MeSH