OBJECTIVE: We evaluated the hypothesis that the development of renal dysfunction and congestive heart failure (CHF) caused by volume overload in rats with angiotensin II (ANG II)-dependent hypertension is associated with altered renal vascular responsiveness to ANG II and to epoxyeicosatrienoic acids (EETs). METHODS: Ren-2 transgenic rats (TGRs) were used as a model of ANG II-dependent hypertension. CHF was induced by volume overload achieved by the creation of the aorto-caval fistula (ACF). Renal blood flow (RBF) responses were determined to renal arterial administration of ANG II, native 11,12-EET, an analog of 14,15-EETs (EET-A), norepinephrine (NE), acetylcholine (Ach) and bradykinin (Bk) in healthy (i.e., sham-operated) TGR and ACF TGR (5 weeks after ACF creation). RESULTS: Selective intrarenal administration of neither vasoactive drug altered mean arterial pressure in any group. Administration of ANG II caused greater decreases in RBF in ACF TGR than in sham-operated TGR, whereas after administration of NE the respective decreases were comparable in the 2 groups. Administration of Ach and Bk elicited significantly higher RBF increases in ACF TGR as compared with sham-operated TGR. In contrast, administration of 11,12-EET and EET-A caused significantly smaller RBF increases in ACF TGR than in sham-operated TGR. CONCLUSION: The findings show that 5 weeks after creation of ACF, the TGR exhibit exaggerated renal vasoconstrictor responses to ANG II and reduced renal vasodilatory responses to EETs, suggesting that both these alterations might play an important role in the development of renal dysfunction in this model of CHF.

• Klíčová slova
• Acetylcholine, Angiotensin II, Aorto-caval fistula, Bradykinin, Congestive heart failure, Epoxyeicosatrienoic acid, Hypertension, Norepinephrine, Renal blood flow, Renal dysfunction, Renal vascular reactivity,
• MeSH
• angiotensin II škodlivé účinky   MeSH
• arteria pulmonalis abnormality   patofyziologie   MeSH
• arterioarteriální píštěl patofyziologie   MeSH
• hypertenze chemicky indukované   komplikace   MeSH
• krysa rodu Rattus MeSH
• potkani transgenní MeSH
• renální oběh účinky léků   MeSH
• srdeční selhání komplikace   patofyziologie   MeSH
• vazodilatace účinky léků   MeSH
• vazokonstrikce účinky léků   MeSH
• vazokonstriktory farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• angiotensin II MeSH
• vazokonstriktory MeSH