Personalized cancer vaccines targeting patient-specific neoantigens are a promising cancer treatment modality; however, neoantigen physicochemical variability can present challenges to manufacturing personalized cancer vaccines in an optimal format for inducing anticancer T cells. Here, we developed a vaccine platform (SNP-7/8a) based on charge-modified peptide-TLR-7/8a conjugates that are chemically programmed to self-assemble into nanoparticles of uniform size (~20 nm) irrespective of the peptide antigen composition. This approach provided precise loading of diverse peptide neoantigens linked to TLR-7/8a (adjuvant) in nanoparticles, which increased uptake by and activation of antigen-presenting cells that promote T-cell immunity. Vaccination of mice with SNP-7/8a using predicted neoantigens (n = 179) from three tumor models induced CD8 T cells against ~50% of neoantigens with high predicted MHC-I binding affinity and led to enhanced tumor clearance. SNP-7/8a delivering in silico-designed mock neoantigens also induced CD8 T cells in nonhuman primates. Altogether, SNP-7/8a is a generalizable approach for codelivering peptide antigens and adjuvants in nanoparticles for inducing anticancer T-cell immunity.

• MeSH
• adjuvancia imunologická chemie   MeSH
• antigeny nádorové imunologie   MeSH
• CD8-pozitivní T-lymfocyty metabolismus   MeSH
• individualizovaná medicína MeSH
• melanom experimentální farmakoterapie   imunologie   MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nanočástice MeSH
• primáti MeSH
• protinádorové vakcíny aplikace a dávkování   imunologie   MeSH
• toll-like receptor 7 imunologie   MeSH
• toll-like receptor 8 imunologie   MeSH
• vakcinace MeSH
• vakcíny konjugované MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Research Support, N.I.H., Intramural MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• adjuvancia imunologická MeSH
• antigeny nádorové MeSH
• protinádorové vakcíny MeSH
• toll-like receptor 7 MeSH
• toll-like receptor 8 MeSH
• vakcíny konjugované MeSH