Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs. In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed. Therefore, our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells.

• MeSH
• fosfatasa 1 s dvojí specificitou metabolismus   MeSH
• fosfatasy s dvojí specifitou genetika   metabolismus   MeSH
• fosfoserin MeSH
• fosfothreonin MeSH
• fosfotyrosin MeSH
• mezoderm metabolismus   MeSH
• mitogenem aktivované proteinkinasy metabolismus   MeSH
• myší embryonální kmenové buňky * metabolismus   MeSH
• myši MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• Dusp7 protein, mouse MeSH Prohlížeč
• fosfatasa 1 s dvojí specificitou MeSH
• fosfatasy s dvojí specifitou MeSH
• fosfoserin MeSH
• fosfothreonin MeSH
• fosfotyrosin MeSH
• mitogenem aktivované proteinkinasy MeSH