Nejvíce citovaný článek - PubMed ID 32321832
MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway
Vacuolar proteins play essential roles in plant physiology and development, but the factors and the machinery regulating their vesicle trafficking through the endomembrane compartments remain largely unknown. We and others have recently identified an evolutionarily conserved plant endosomal sorting complex required for transport (ESCRT)-associated protein apoptosis-linked gene-2 interacting protein X (ALIX), which plays canonical functions in the biogenesis of the multivesicular body/prevacuolar compartment (MVB/PVC) and in the sorting of ubiquitinated membrane proteins. In this study, we elucidate the roles and underlying mechanism of ALIX in regulating vacuolar transport of soluble proteins, beyond its conventional ESCRT function in eukaryotic cells. We show that ALIX colocalizes and physically interacts with the retromer core subunits Vps26 and Vps29 in planta. Moreover, double-mutant analysis reveals the genetic interaction of ALIX with Vps26 and Vps29 for regulating trafficking of soluble vacuolar proteins. Interestingly, depletion of ALIX perturbs membrane recruitment of Vps26 and Vps29 and alters the endosomal localization of vacuolar sorting receptors (VSRs). Taken together, ALIX functions as a unique retromer core subcomplex regulator by orchestrating receptor-mediated vacuolar sorting of soluble proteins.
- Klíčová slova
- ESCRT machiner, endosomal recycling, multivesicular body/prevacuolar compartment (MVB/PVC), retromer complex, vacuolar trafficking,
- MeSH
- Arabidopsis * metabolismus MeSH
- endozomální třídící komplexy pro transport metabolismus MeSH
- endozomy metabolismus MeSH
- proteiny huseníčku * genetika metabolismus MeSH
- rostliny metabolismus MeSH
- transport proteinů fyziologie MeSH
- transportní proteiny metabolismus MeSH
- vakuoly metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ALIX protein, Arabidopsis MeSH Prohlížeč
- endozomální třídící komplexy pro transport MeSH
- proteiny huseníčku * MeSH
- transportní proteiny MeSH
