This study deals with utilization of the hyaluronic acid (HA) and carbonyl iron (CI) microparticles to fabricate the magneto-responsive hydrogel scaffolds that can provide triggered functionality upon application of an external magnetic field. The various combinations of the HA and CI were investigated from the rheological and viscoelastic point of view to clearly show promising behavior in connection to 3D printing. Furthermore, the swelling capabilities with water diffusion kinetics were also elucidated. Magneto-responsive performance of bulk hydrogels and their noncytotoxic nature were investigated,, and all hydrogels showed cell viability in the range 75-85%. The 3D printing of such developed systems was successful, and fundamental characterization of the scaffolds morphology (SEM and CT) has been presented. The magnetic activity of the final scaffolds was confirmed at a very low magnetic field strength of 140 kA/m, and such a scaffold also provides very good biocompatibility with NIH/3T3 fibroblasts.

• Klíčová slova
• 3D printing, hyaluronic acid, magnetic particles, magneto-responsive, scaffold,
• MeSH
• 3D tisk * MeSH
• biokompatibilní materiály * chemie   farmakologie   MeSH
• biopolymery chemie   MeSH
• buňky NIH 3T3 MeSH
• hydrogely chemie   farmakologie   MeSH
• karbonylové sloučeniny železa chemie   MeSH
• kyselina hyaluronová * chemie   farmakologie   MeSH
• myši MeSH
• testování materiálů * MeSH
• tkáňové podpůrné struktury * chemie   MeSH
• velikost částic * MeSH
• viabilita buněk * účinky léků   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biokompatibilní materiály * MeSH
• biopolymery MeSH
• hydrogely MeSH
• karbonylové sloučeniny železa MeSH
• kyselina hyaluronová * MeSH

This work explores application of phase separation phenomena for structuring of films made from hyaluronan. A time-sequenced dispensing of different solution mixtures was applied under rotation of hyaluronan-covered substrates to generate surface textures. This method is applicable in direct surface modification or cover layer deposition. Changes in the surface topography were characterized by atomic force microscopy, optical microscopy, and contact and non-contact profilometry. The mechanical properties of the surface-modified self-supporting films were compared using a universal testing machine. Experimental results show that diverse hyaluronan-based surface reliefs and self-supporting films with improved mechanical properties can be prepared using a newly designed multi-step phase separation process without the need for sacrificial removable templates or additives.

• Publikační typ
• časopisecké články MeSH