This work explores application of phase separation phenomena for structuring of films made from hyaluronan. A time-sequenced dispensing of different solution mixtures was applied under rotation of hyaluronan-covered substrates to generate surface textures. This method is applicable in direct surface modification or cover layer deposition. Changes in the surface topography were characterized by atomic force microscopy, optical microscopy, and contact and non-contact profilometry. The mechanical properties of the surface-modified self-supporting films were compared using a universal testing machine. Experimental results show that diverse hyaluronan-based surface reliefs and self-supporting films with improved mechanical properties can be prepared using a newly designed multi-step phase separation process without the need for sacrificial removable templates or additives.

Centre of Polymer Systems Tomas Bata University in Zlín Třída Tomáše Bati 5678 Zlín 760 01 Czech Republic

Department of Biomaterials Faculty of Engineering Science Universität Bayreuth Prof Rüdiger Bormann Str 1 Bayreuth 95447 Germany

Department of Physics and Materials Engineering Tomas Bata University in Zlín Vavrečkova 5669 Zlín 760 01 Czech Republic

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