The enantioselective potential of two macrocyclic glycopeptide-based chiral stationary phases for analysis of 28 structurally diverse biologically active compounds such as derivatives of pyrovalerone, ketamine, cathinone, and other representatives of psychostimulants and antidepressants was evaluated in sub/supercritical fluid chromatography. The chiral selectors immobilized on 2.7 μm superficially porous particles were teicoplanin (TeicoShell column) and modified macrocyclic glycopeptide (NicoShell column). The influence of the organic modifier and different mobile phase additives on the retention and enantioresolution were investigated. The obtained results confirmed that the mobile phase additives, especially water as a single additive or in combination with basic and acidic additives, improve peak shape and enhance enantioresolution. In addition, the effect of temperature was evaluated to optimize the enantioseparation process. Both columns exhibited comparable enantioselectivity, approximately 90% of the compounds tested were enantioseparated, and 30% out of them were baseline enantioresolved under the tested conditions. The complementary enantioselectivity of the macrocyclic glycopeptide-based chiral stationary phases was emphasized. This work can be useful for the method development for the enantioseparation of basic biologically active compounds of interest.

• Keywords
• NicoShell, SFC, TeicoShell, basic bioactive compounds, enantioseparation, macrocyclic glycopeptides, superficially porous particles,
• MeSH
• Glycopeptides chemistry   MeSH
• Pharmaceutical Preparations MeSH
• Stereoisomerism MeSH
• Chromatography, Supercritical Fluid * methods   MeSH
• Teicoplanin chemistry   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glycopeptides MeSH
• Pharmaceutical Preparations MeSH
• Teicoplanin MeSH

This review provides a brief survey of chiral separation of pharmaceutically active substances published over the last 3 years (2018-2020). Chiral separation of drugs is an important area of research. The control of enantiomeric purity and determination of individual enantiomeric drug molecules is a necessity especially for clinical, analytical, and regulatory purposes. Among chromatographic resolution methods, high-performance liquid chromatography based on chiral stationary phases remains the most popular and effective method used for chiral separation of various drugs. In this review, attention is paid to several classes of chiral stationary phases that have been the most frequently used for drug enantioseparation during this period.

• Keywords
• Chiral resolution, Chiral stationary phase, HPLC, Pharmaceutical compounds,
• Publication type
• Journal Article MeSH
• Review MeSH