Due to excellent mechanical properties and biocompatibility, materials based on silk fibroin are increasingly included in advanced biomedical research and applications. However, their poor supporting properties for cell adhesion and proliferation represent limiting factors of the utilization. To eliminate this deficiency, we developed a series of phase-separation approaches allowing for tunable texturing of planar and 3D printed fibroin surfaces from nano to macro levels. The formation of surface structures presented is based on a combination of good and poor solvents, whereas no potentially problematic templates or additives, diminishing biocompatibility of the resulting material, are required. A critical factor in obtaining and scaling of the textures is control over the degree of transformation of fibroin secondary structures between prevalently amorphous Silk I and semicrystalline Silk II forms before and during surface treatment. Employing a set of optimized procedures, selectively or hierarchically structured fibroin surfaces can be prepared at the nano, micro, and macro level, which are characterized by long-term stability in physiological environments, allowing enhanced adhesion and proliferation of human keratinocytes as well as skin fibroblast cultivations.

• Klíčová slova
• 3D printing, cell interaction, phase separation, secondary structure, silk fibroin, surface texture,
• MeSH
• biokompatibilní materiály * chemie   farmakologie   MeSH
• buněčná adheze účinky léků   MeSH
• fibroblasty cytologie   MeSH
• fibroiny * chemie   farmakologie   MeSH
• keratinocyty cytologie   účinky léků   MeSH
• lidé MeSH
• povrchové vlastnosti MeSH
• proliferace buněk účinky léků   MeSH
• separace fází MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biokompatibilní materiály * MeSH
• fibroiny * MeSH