This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

• Klíčová slova
• Ag-NPs, Au-NPs, Escherichia coli, SERS, Staphylococcus aureus, phenylalanine,
• Publikační typ
• časopisecké články MeSH

The feature issue of Biomedical Optics Express presents studies that were the focus of the Optical Manipulation and its Applications (OMA) meeting that was held on 24 - 27 April 2022 in Vancouver, Canada.

• Publikační typ
• časopisecké články MeSH