Staphylococcus pseudintermedius is a species often isolated from animals, as a common element of their microbiota or an agent of infection, and from people associated with an animal habitat, including owners of home pets-dogs and cats. As with many other species, adaptation of these bacteria to the human body can occur, and they become important human pathogens. 59 S. pseudintermedius strains were investigated in this study to determine the factors contributing to human body colonization: inhibition growth of human skin residents isolated from human skin (Staphylococcus epidermidis, Corynebacterium spp., Cutibacterium acnes (formerly Propionibacterium acnes)), biofilm formation, and the presence of ten genes encoding infection-promoting features (including ebpS, spsE, lukS, lukF, pvl, lip, hlgA, hlgB). The ability of human skin to be colonized and the presence of genes that promote the development of skin infections showed the significant potential of the studied strains in their adaptation to the host. However, while a comparison of the characteristics of animal strains and those isolated from human infections does not allow us to claim that we are the witnesses of the speciation of a new human pathogen, it does indicate their gradual adaptation to the human organism.

• Keywords
• Colonization, Companion animals, Skin microflora, Staphylococcus pseudintermedius, Virulence,
• MeSH
• Genes, Bacterial MeSH
• Phenotype MeSH
• Cats MeSH
• Humans MeSH
• Microbiota MeSH
• Dogs MeSH
• Staphylococcal Infections microbiology   transmission   MeSH
• Staphylococcus classification   physiology   MeSH
• Virulence MeSH
• Zoonoses microbiology   MeSH
• Animals MeSH
• Check Tag
• Cats MeSH
• Humans MeSH
• Dogs MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

Numerous isolates of both subspecies of Staphylococcus cohnii were found in the environment of the intensive-care unit of a pediatric hospital. These isolates carried in their cells many plasmids, up to fourteen, of a wide range of sizes (< 2 to > 56 kb). Striking was the occurrence of large plasmids not very common in staphylococci. These were present in > 80% of S. cohnii isolates. Fifty-two different plasmid profiles were found in 79 investigated isolates belonging to S. cohnii ssp. cohnii and S. cohnii ssp. urealyticus. Isolates similar in plasmid profiles were grouped in antibiotic-resistance clusters established for 9 antibiotics (gentamicin, ciprofloxacin, clindamycin, erythromycin, tetracycline, chloramphenicol, mupirocin, trimethoprim-sulfamethoxazole, vancomycin) using the method of unweighted pair group mathematical averages (UPGMA). Many isolates were multiresistant to antibiotics and produced bacteriocins.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Drug Resistance, Bacterial genetics   MeSH
• Bacteriocins biosynthesis   MeSH
• Chloramphenicol pharmacology   MeSH
• Ciprofloxacin pharmacology   MeSH
• Hospitals, Pediatric * MeSH
• DNA, Bacterial analysis   isolation & purification   MeSH
• DNA Fingerprinting MeSH
• Erythromycin pharmacology   MeSH
• Gentamicins pharmacology   MeSH
• Intensive Care Units * MeSH
• Clindamycin pharmacology   MeSH
• Trimethoprim, Sulfamethoxazole Drug Combination pharmacology   MeSH
• Microbial Sensitivity Tests MeSH
• Drug Resistance, Multiple, Bacterial genetics   MeSH
• Molecular Epidemiology MeSH
• Mupirocin pharmacology   MeSH
• Plasmids * MeSH
• Staphylococcus classification   drug effects   genetics   isolation & purification   MeSH
• Bacterial Typing Techniques MeSH
• Tetracycline pharmacology   MeSH
• Vancomycin pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Poland MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Bacteriocins MeSH
• Chloramphenicol MeSH
• Ciprofloxacin MeSH
• DNA, Bacterial MeSH
• Erythromycin MeSH
• Gentamicins MeSH
• Clindamycin MeSH
• Trimethoprim, Sulfamethoxazole Drug Combination MeSH
• Mupirocin MeSH
• Tetracycline MeSH
• Vancomycin MeSH