Nejvíce citovaný článek - PubMed ID 4136216
Electronmicroscopic comparison of the acid-fast and non-acid-fast strain of Mycobacterium phlei
Gene manipulation in mycobacteria developed in two phases. In the first phase genes of mycobacteria were transferred into cells of E. coli and Streptomyces lividans. In the second phase, heterologous genes were transferred into mycobacteria either with a shuttle plasmid or hybrid plasmids. A prerequisite for successful gene manipulation in mycobacteria was a thorough understanding of plasmids in mycobacteria. Construction of recombinant DNA molecules contributed not only to the fact that mycobacteria did not remain outside the mainstream of modern genetic research but also to their present practical importance.
Nalidixic acid was used for describing more accurately the terminal replication region of the Mycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185-190 min, i.e. about 10 min after replication of the ser gene, the last of 24 genes of the replication map described so far. The replication of the chromosome was controlled by determining the position of the bac gene. Microscopic studies in phase contrast of the cells that were subjected for long time periods to nalidixic acid treatment at a bactericidal concentration showed elongated cells. The electronmicroscopic observation showed that a portion of the population influenced by nalidixic acid lyses, whereas other cells remain intact and resemble control cells.
- MeSH
- bakteriální chromozomy účinky léků metabolismus MeSH
- buněčné dělení účinky léků MeSH
- DNA bakterií biosyntéza MeSH
- kyselina nalidixová farmakologie MeSH
- mapování chromozomů * MeSH
- Mycobacterium phlei účinky léků genetika růst a vývoj MeSH
- Mycobacterium genetika MeSH
- replikace DNA účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA bakterií MeSH
- kyselina nalidixová MeSH
