Substances studied at this department in 1954-1983 are reviewed; a total of 226 compounds are characterized in a tabular form. They include natural compounds as well as those prepared by biotransformation, by semisynthetic and synthetic methods.

• MeSH
• Anti-Bacterial Agents isolation & purification   MeSH
• Microbiology MeSH
• Chemistry, Organic * MeSH
• Organic Chemistry Phenomena MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH

Srimivasan's coagulation test was performed in 18 strains of the genus Streptomyces and one strain of the genus Actinoplanes. The highest coagulation activity was detected in strains systematically classified in a series of streptomycetes with pink or red aerial mycelium: S. erythreus, Streptomyces sp. AJ/22, S. roseo-luteus and S. griseofuscus. With the exception of S. griscofuscus these three cultures also exhibited the highest inhibitory activity B. subtilis. When using hemoglobin as substrate it was possible to detect acid, neutral and alkaline proteinases with the highest poroteolytic activity at pH 3.0 to 4.0 in the most active strain of S. erythreus.

• MeSH
• Actinomycetales cytology   enzymology   MeSH
• Anti-Bacterial Agents biosynthesis   MeSH
• Bacteriological Techniques MeSH
• Species Specificity MeSH
• Milk MeSH
• Peptide Hydrolases metabolism   MeSH
• Streptomyces classification   cytology   enzymology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Peptide Hydrolases MeSH

The strain designated Actinoplanes sp. 220 differed in its characteristics from other strains of the genus Actinoplanes listed in Bergey's Manual (1974). The strain belongs to psychrophilic culture growing within the range of 0-30 degrees C. The optimal temperature for growth on yeast--malt agar is 10-23 degrees C. Cultures transferred at 23 and 28 degrees C differed in morphological and physiological properties, enzyme activity and pigmentation in standard media. Submerged culture transferred at 28 degrees C inhibited growth of Bacillus subtilis ATCC 6633 and ATCC 9945. LL-2,6-Diaminopimelic acid was chromatographically detected in the submerged mycelium of this culture. This compound was not found in the mycelium of the original culture transferred at 23 degrees C. The cultures did not substantially differ in the composition of other amino acids contained in larger quantities in the mycelium.

• MeSH
• Actinomycetales classification   growth & development   metabolism   MeSH
• Culture Media MeSH
• Diaminopimelic Acid metabolism   MeSH
• Cold Temperature * MeSH
• Chromatography, Paper MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Culture Media MeSH
• Diaminopimelic Acid MeSH

The interaction of granaticin B, a quinone antibiotic produced by Streptomyces granaticolor, with some biologically important bivalent metal ions, DNA and ATP was demonstrated spectrophotometrically. The activity of isolated RNA polymerase was higher when the DNA of phage SP 50 served as template than with DNA isolated from Bacillus subtilis. Granaticin B inhibited in vitro RNA synthesis, similarly to certain other antibiotics (the inhibition was three times lower than that caused by actinomycin D or streptolydigin and slightly higher than that by epsilon-pyrromycinone). The inhibitory effect was higher when the Mg2+ concentration in the reaction mixture was decreased. The inhibition was then proportional to the concentration of the DNA template. DNA-dependent RNA synthesis is thus inhibited in vitro by granaticin B but this does not appear to be the only site of action of this antibiotic in vivo.

• MeSH
• Adenosine Triphosphate MeSH
• Bacillus subtilis growth & development   metabolism   MeSH
• RNA, Bacterial biosynthesis   MeSH
• Bacteriophages MeSH
• Benzofurans pharmacology   MeSH
• DNA-Directed RNA Polymerases metabolism   MeSH
• DNA MeSH
• Transcription, Genetic drug effects   MeSH
• Templates, Genetic MeSH
• Magnesium MeSH
• Spectrophotometry MeSH
• Terpenes pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Adenosine Triphosphate MeSH
• RNA, Bacterial MeSH
• Benzofurans MeSH
• DNA-Directed RNA Polymerases MeSH
• DNA MeSH
• Magnesium MeSH
• Terpenes MeSH

• MeSH
• Anti-Bacterial Agents biosynthesis   MeSH
• Pigments, Biological biosynthesis   MeSH
• Carbohydrate Metabolism MeSH
• Streptomyces classification   cytology   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Pigments, Biological MeSH