Spores of Bacillus cereus were germinated in a germination limited medium (GL-medium) which facilitates only germination but not the postgerminative development of spores. Under these conditions a limited protein synthesis occurs. However, this protein synthesis is stopped after a short time interval. The rate of synthesis of new proteins, as well as their total amount, is influenced by the length of the activation heat shock. Synthesis of the wall material continues for several hours and thick-walled cells with a changed ultrastructure are formed. Synthesis of the diaminopimelic acid (dap) containing material of the cell wall is sensitive to actinomycin D and relatively resistant to chloramphenicol. Similarly, protein synthesis is relatively chlorapmhenicol-resistant but is fully inhibited by azauracil or spiramycin. Whereas RNA formed in the control culture is partially decomposed after 30 min of incubation, chloramphenicol accelerates its synthesis and prevents its decay. Exudate components apparently stimulate synthesis of ribonucleic acid, proteins and the wall material. The 14-C-dap containing material released by prelabelled spores in the form of the exudate during the germination is not re-utilized by the spores germinated in the GL-medium. The results are discussed with respect to the atypical primary synthetic activities of spores under conditions when the postgerminative development is prevented and from the point of view of participation of the germination exudate during these syntheses.

• MeSH
• Bacillus cereus drug effects   metabolism   MeSH
• Bacterial Proteins biosynthesis   MeSH
• RNA, Bacterial biosynthesis   metabolism   MeSH
• Cell Wall drug effects   metabolism   MeSH
• Chloramphenicol pharmacology   MeSH
• Dactinomycin pharmacology   MeSH
• Kinetics MeSH
• Culture Media MeSH
• Leucomycins pharmacology   MeSH
• Protein Precursors MeSH
• Spores, Bacterial drug effects   growth & development   metabolism   MeSH
• Uracil analogs & derivatives   pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Proteins MeSH
• RNA, Bacterial MeSH
• Chloramphenicol MeSH
• Dactinomycin MeSH
• Culture Media MeSH
• Leucomycins MeSH
• Protein Precursors MeSH
• Uracil MeSH

• MeSH
• Bacillus cereus drug effects   metabolism   MeSH
• Bacterial Proteins biosynthesis   MeSH
• RNA, Bacterial metabolism   MeSH
• Histones pharmacology   MeSH
• Culture Media MeSH
• Leucine metabolism   MeSH
• Macromolecular Substances MeSH
• Ribosomes metabolism   MeSH
• Spores, Bacterial drug effects   MeSH
• Binding Sites MeSH
• Germ Cells drug effects   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Proteins MeSH
• RNA, Bacterial MeSH
• Histones MeSH
• Culture Media MeSH
• Leucine MeSH
• Macromolecular Substances MeSH

• MeSH
• Bacillus * MeSH
• Cell Differentiation * MeSH
• Spores MeSH
• Publication type
• Journal Article MeSH
• Review MeSH

• MeSH
• Bacillus cereus drug effects   MeSH
• Carbon Isotopes MeSH
• Leucine metabolism   MeSH
• Lysine pharmacology   MeSH
• Polymyxins pharmacology   MeSH
• Protamines pharmacology   MeSH
• Spores drug effects   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Carbon Isotopes MeSH
• Leucine MeSH
• Lysine MeSH
• Polymyxins MeSH
• Protamines MeSH

• MeSH
• Bacillus cereus growth & development   MeSH
• Bacterial Proteins biosynthesis   MeSH
• RNA, Bacterial biosynthesis   MeSH
• Sulfur Isotopes analysis   MeSH
• Carbon Isotopes analysis   MeSH
• Leucine metabolism   MeSH
• Methionine metabolism   MeSH
• Spores MeSH
• Uracil metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Proteins MeSH
• RNA, Bacterial MeSH
• Sulfur Isotopes MeSH
• Carbon Isotopes MeSH
• Leucine MeSH
• Methionine MeSH
• Uracil MeSH

• MeSH
• Bacillus cereus growth & development   MeSH
• RNA, Bacterial biosynthesis   MeSH
• Cell Membrane * MeSH
• Chromatography MeSH
• Cycloserine metabolism   MeSH
• Culture Media MeSH
• Trichloroacetic Acid metabolism   MeSH
• Pimelic Acids metabolism   MeSH
• RNA, Messenger * MeSH
• Methionine metabolism   MeSH
• Penicillins metabolism   MeSH
• Radioisotopes MeSH
• Spores MeSH
• In Vitro Techniques MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• RNA, Bacterial MeSH
• Cycloserine MeSH
• Culture Media MeSH
• Trichloroacetic Acid MeSH
• Pimelic Acids MeSH
• RNA, Messenger * MeSH
• Methionine MeSH
• Penicillins MeSH
• Radioisotopes MeSH