This review summarizes the main results obtained in the fields of general and molecular microbiology and microbial genetics at the Institute of Microbiology of the Academy of Sciences of the Czech Republic (AS CR) [formerly Czechoslovak Academy of Sciences (CAS)] over more than 50 years. Contribution of the founder of the Institute, academician Ivan Málek, to the introduction of these topics into the scientific program of the Institute of Microbiology and to further development of these studies is also included.

• MeSH
• Academies and Institutes history   MeSH
• History, 20th Century MeSH
• Genetics, Microbial history   MeSH
• Molecular Biology history   MeSH
• Check Tag
• History, 20th Century MeSH
• Publication type
• Journal Article MeSH
• Historical Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH
• Geographicals
• Czech Republic MeSH

Results obtained when studying conjugation in mycobacteria by means of different methods are summarized. The method of conjugation on surface of a solid complete medium was tested with different auxotrophic mutants of different strains of Mycobacterium smegmatis. It was not possible to obtain positive results even by means of the above method. This was probably due to unsuitability of the chosen strains of Mycobacterium smegmatis. Preparation of the donor strain by transfer of the F factor from Escherichia coli F'ORF 1 ade+ lac+ pro+ to Mycobacterium phlei PA ade Stmr by means of sexduction is described. Frequency of the phenotype PA ade+ Stmr increased in the average by two and a half orders of magnitude with respect to the control, however, a further transfer from cultures of the cells ade+ Stmr to cells ade could not be demonstrated. Experiments aimed at transferring the R factor from strains Escherichia coli K-12 to Mycobacterium phlei were unsuccessful.

• MeSH
• Escherichia coli MeSH
• F Factor MeSH
• Conjugation, Genetic * MeSH
• Culture Media MeSH
• Methylnitronitrosoguanidine MeSH
• Mutation MeSH
• Mutagens MeSH
• Mycobacterium phlei growth & development   MeSH
• Mycobacterium * growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Culture Media MeSH
• Methylnitronitrosoguanidine MeSH
• Mutagens MeSH

• MeSH
• Alanine analysis   MeSH
• Amino Sugars analysis   MeSH
• Amino Acids analysis   MeSH
• Autoanalysis MeSH
• Bacterial Proteins analysis   MeSH
• Staining and Labeling MeSH
• Cell Membrane MeSH
• Cell Wall analysis   drug effects   MeSH
• Cytoplasm MeSH
• Microscopy, Electron MeSH
• Glutamates analysis   MeSH
• Glycine pharmacology   MeSH
• Pimelic Acids analysis   MeSH
• Acids MeSH
• Mucoproteins analysis   MeSH
• Muramidase pharmacology   MeSH
• Mutation * MeSH
• Mycobacterium analysis   cytology   MeSH
• Chromatography, Paper MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• Alanine MeSH
• Amino Sugars MeSH
• Amino Acids MeSH
• Bacterial Proteins MeSH
• Glutamates MeSH
• Glycine MeSH
• Pimelic Acids MeSH
• Acids MeSH
• Mucoproteins MeSH
• Muramidase MeSH