When studying the biosynthesis of mucidin under production (glucose as the main carbon source) and non-production (glucitol as the main carbon source) conditions it could be shown that the producer, Oudemansiella mucida, utilizes glucitol both for growth and for mucidin biosynthesis. However, the production of mucidin is 10 times lower than on glucose. When the culture was preincubated on glucose and transferred to non-production conditions the negative effect of glucitol could not be demonstrated. Biosynthesis of mucidin is influenced by the used carbon source already at an early stage of the cultivation.

• MeSH
• Agaricales účinky léků   růst a vývoj   metabolismus   MeSH
• antibakteriální látky biosyntéza   MeSH
• glukosa metabolismus   MeSH
• kultivační média metabolismus   MeSH
• methakryláty MeSH
• nenasycené mastné kyseliny MeSH
• sorbitol metabolismus   farmakologie   MeSH
• strobiluriny MeSH
• uhlík metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• glukosa MeSH
• kultivační média MeSH
• methakryláty MeSH
• mucidin MeSH Prohlížeč
• nenasycené mastné kyseliny MeSH
• sorbitol MeSH
• strobiluriny MeSH
• uhlík MeSH

Mutants of Oudemansiella mucida, blocked in the biosynthesis of the antibiotic mucidin, were obtained at a 0.28% frequency after the application of N-methyl-N'-nitro-N-nitrosoguanidine (MNG) to basidiospores under conditions leading to 0.5--5.0% survival rates. Loss of antibiotic activity was in most isolates accompanied by a decrease in mycelium growth rate and a suppression of dikaryotizing and fructification ability. Recombination analysis of two stable mutants revealed that the block in mucidin synthesis is the result of mutation in the same chromosomal gene (muc). In contrast to the action of MNG, UV-irradiation leads neither to the loss of biosynthetic activity nor to any morphological change.

• MeSH
• Agaricales genetika   metabolismus   fyziologie   MeSH
• antibakteriální látky biosyntéza   MeSH
• geny * MeSH
• křížení genetické MeSH
• methakryláty MeSH
• methylnitronitrosoguanidin MeSH
• mutace MeSH
• nenasycené mastné kyseliny MeSH
• strobiluriny MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• methakryláty MeSH
• methylnitronitrosoguanidin MeSH
• mucidin MeSH Prohlížeč
• nenasycené mastné kyseliny MeSH
• strobiluriny MeSH