The history of the Czech and Slovak experimental cardiology describes a completely unusual curve. The personality of J.E. Purkynje caused this field to reach unprecedented peak at the very beginning of its modern history. The development of experimental cardiology after the death of the great scholar was certainly not linear. Just when it seemed to be raising its head, the German occupation came. Its second hopeful awakening was delayed for a long time by forty years of isolation. The significant limitation of foreign contacts gradually led to the loss of hopefully developing contacts, to professional isolation and lagging behind the stormy development of world science. At the moment of greatest depression, in 1971, in Prague there was created a professional forum that was supposed to enable its intellectual survival and reduce the negative consequences of the "splendid isolation". The Society of Experimental Cardiology (SEC) was founded at the Czechoslovak Physiological Society of the Czechoslovak Medical Society J.E. Purkynje, with the main task of introducing theoretical and clinical cardiologists to the advances in world cardiology. The first meeting was held in 1973 and in 2023 we celebrated already the 50th anniversary of SEC. Moreover, nowadays we see the increasing interest of the young researchers, both experimental and clinical cardiologists, who consider SEC a very attractive platform for their education and professional growth. Key words: Experimental cardiology, Czech and Slovak Society, History, Relationship to clinical cardiology.

• MeSH
• biomedicínský výzkum * dějiny   MeSH
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• kardiologie * dějiny   MeSH
• lidé MeSH
• společnosti lékařské * dějiny   MeSH
• Check Tag
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• Geografické názvy
• Česká republika MeSH
• Slovenská republika MeSH

The aim of the study was to find out whether administration of selenium (Se) will protect the immature heart against ischemia/reperfusion.The control pregnant rats were fed laboratory diet (0.237 mg Se/kg diet); experimental rats received 2 ppm Na(2)SeO(3) in the drinking water from the first day of pregnancy until day 10 post partum. The concentration of Se in the serum and heart tissue was determined by activation analysis, the serum concentration of NO by chemiluminescence, cardiac concentration of lipofuscin-like pigment by fluorescence analysis. The 10 day-old hearts were perfused (Langendorff); recovery of developed force (DF) was measured after 40 min of global ischemia. In acute experiments, 10 day-old hearts were perfused with selenium (75 nmol/l) before or after global ischemia. Sensitivity to isoproterenol (ISO, pD(50)) was assessed as a response of DF to increasing cumulative dose.Se supplementation elevated serum concentration of Se by 16%. Se increased ischemic tolerance (recovery of DF, 32.28 +/- 2.37 vs. 41.82 +/- 2.91%, P < 0.05). Similar results were obtained after acute administration of Se during post-ischemic reperfusion (32.28 +/- 2.37 vs. 49.73 +/- 4.40%, P < 0.01). The pre-ischemic treatment, however, attenuated the recovery (23.08 +/- 3.04 vs. 32.28 +/- 2.37%, P < 0.05). Moreover, Se supplementation increased the sensitivity to the inotropic effect of ISO, decreased cardiac concentration of lipofuscin-like pigment and serum concentration of NO. Our results suggest that Se protects the immature heart against ischemia/reperfusion injury. It seems therefore, that ROS may affect the function of the neonatal heart, similarly as in adults.

• MeSH
• časové faktory MeSH
• ischemická choroba srdeční farmakoterapie   prevence a kontrola   MeSH
• kontrakce myokardu účinky léků   MeSH
• krysa rodu Rattus MeSH
• lipofuscin metabolismus   MeSH
• oxid dusnatý krev   MeSH
• perfuze MeSH
• potkani Wistar MeSH
• potravní doplňky MeSH
• reperfuzní poškození myokardu farmakoterapie   prevence a kontrola   MeSH
• selen krev   farmakologie   terapeutické užití   MeSH
• srdce účinky léků   MeSH
• těhotenství MeSH
• tělesná hmotnost účinky léků   MeSH
• velikost orgánu účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• lipofuscin MeSH
• oxid dusnatý MeSH
• selen MeSH

The function of the hypertrophic right ventricle (RV) was studied in adult rats with hypoxic pulmonary hypertension induced by intermittent high-altitude (IHA) exposure. The isolated RV working heart preparation that was employed enabled us to estimate ventricular contractile and pump performance under controlled loading conditions. In rats exposed to IHA hypoxia the elevated RV systolic pressure and maximum rate of pressure development were observed at various levels of preload or afterload. The peak indices of mechanical performance were almost doubled in these animals when compared with the normoxic group, while the index of contractility remained unchanged. Maximum ventricular performance was found to be a linear function of the relative RV weight. No evidence of RV pump dysfunction was detected in rats exposed to IHA; moreover, the ability of the ventricle to maintain cardiac output against increased pulmonary resistance was markedly improved. The prevention of tricuspid regurgitation by using an artificial valve did not influence the functional curves and the peak ventricular performance. The regression of hypertrophy was accompanied by a reversal of ventricular function to control values, except for the persisting slight increase of peak RV pressure. It may be concluded that the increase of the RV mass in IHA-exposed rats serves to improve maximum ventricular performance, which aids in overcoming and elevated pulmonary resistance without disturbing the pump function.

• MeSH
• inbrední kmeny potkanů MeSH
• kardiomegalie patofyziologie   MeSH
• krevní tlak MeSH
• krysa rodu Rattus MeSH
• plicní hypertenze komplikace   patofyziologie   MeSH
• regresní analýza MeSH
• tělesná hmotnost MeSH
• velikost orgánu MeSH
• výšková nemoc komplikace   patofyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH