Position of cells in their cell cycle was determined microscopically in chemostat cultures of Candida utilis. Proportion of cells in phase G1 decreased in a linear manner from 86% to 58% with dilution rate. Proportion of cells in phase S increased in the same range of D from 5.6 to 13.5% and in the (G2 + M) phase from 8.4 to 28.5%, again linearly. Differential centrifugation was used to separate chemostat cultures to mother and daughter cells. Analyses showed that, relative to mother cells, daughter cells contain 2.1-11.9% more protein and 25.5-34.6% more RNA in dry matter. Their mass is 34.4-5.6% lower and volume is 154-19% smaller.

• MeSH
• Cell Cycle * MeSH
• Candida analysis   cytology   growth & development   MeSH
• Centrifugation MeSH
• Fungal Proteins analysis   MeSH
• RNA, Fungal analysis   MeSH
• Interphase MeSH
• Mitosis MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Fungal Proteins MeSH
• RNA, Fungal MeSH

When investigating changes of respiratory activity during the cell cycle of mother and daughter Candida cells significant oscillations of specific rate of oxygen consumption were detected; specific growth rate also varied. The oscillations were less pronounced when the inoculum was obtained from the chemostat at the high dilution rates of 0.25 and 0.35/h.

• MeSH
• Models, Biological MeSH
• Cell Cycle * MeSH
• Candida growth & development   metabolism   physiology   MeSH
• Energy Metabolism MeSH
• Glucose metabolism   MeSH
• Interphase MeSH
• Oxygen Consumption * MeSH
• Cell Survival MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glucose MeSH