Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis of CsCl gradient-purified VLPs on PAGE/SDS electrophoresis showed one major protein component with an apparent molecular weight of 75 kDa.

• MeSH
• RNA, Double-Stranded genetics   MeSH
• Microscopy, Electron MeSH
• Fungi genetics   MeSH
• Molecular Weight MeSH
• Polymorphism, Genetic MeSH
• RNA Viruses chemistry   genetics   isolation & purification   ultrastructure   MeSH
• Viral Proteins chemistry   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• RNA, Double-Stranded MeSH
• Viral Proteins MeSH

The anomeric specificity of monosaccharide uptake was investigated in 42 species of yeasts and related mycelium-forming fungi. Differences in the uptake of anomers were determined by the following methods. (1) Shift of anomeric equilibrium in the outer medium caused by preferential uptake of one of the anomeric forms was monitored polarimetrically as induced mutarotation. (2) The uptake of 14C-D-glucose by cells was examined after addition of freshly prepared solutions of alpha- or beta-D-glucose. Most of the organisms examined display the Saccharomyces-type preference for the alpha-anomers of glucose and xylose which is caused by the higher affinity of the monosaccharide carrier for the alpha-pyranose configuration. The following genera show this type of preference (the number of species is given in parenthesis): Saccharomyces (5), Schizosaccharomyces (1), Endomycopsis (2), Eremascus (1), Endomyces (1), Pichia (1), Hansenula (1), Debaryomyces (2), Lipomyces (1), Willia (1), Nematospora (1), Kluyveromyces (2), Candida (5), Torulopsis (5), Cryptococcus (1). No anomeric specificity was shown by the following genera: Nadsonia (1), Dipodascus (2), Rhodotorula (5), Sporobolomyces (2), Bullera (1), Rhodosporidium (1). A parallel investigation of the concentration dependence of glucose uptake indicates that most yeasts possess a constitutive monosaccharide carrier characterized by the following features: a high maximum rate of uptake, a relatively low affinity, and preference for alpha-anomers. Besides this carrier the majority of these microorganisms possess a glucose-transporting carrier with a higher affinity and a lower capacity.

• MeSH
• Biological Transport, Active MeSH
• Species Specificity MeSH
• Glucose metabolism   MeSH
• Fungi metabolism   MeSH
• Yeasts metabolism   MeSH
• Monosaccharides metabolism   MeSH
• Monosaccharide Transport Proteins MeSH
• Stereoisomerism MeSH
• Carrier Proteins metabolism   MeSH
• Xylose metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glucose MeSH
• Monosaccharides MeSH
• Monosaccharide Transport Proteins MeSH
• Carrier Proteins MeSH
• Xylose MeSH