Nejvíce citovaný článek - PubMed ID 7141331
Regulation of biosynthesis of monensins on chemically defined medium
Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.
- MeSH
- glycin MeSH
- kultivační média MeSH
- muramidasa MeSH
- mutace MeSH
- mykologie metody MeSH
- protoplasty cytologie fyziologie MeSH
- Streptomyces cytologie genetika fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glycin MeSH
- kultivační média MeSH
- muramidasa MeSH
A total of 41 compounds studied at the Department of Biogenesis of Natural Substances between 1984 and 1988 are characterized and tabulated. They include natural, semisynthetic and synthetic compounds.
- MeSH
- Bacteria metabolismus MeSH
- monensin izolace a purifikace MeSH
- námelové alkaloidy izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- monensin MeSH
- námelové alkaloidy MeSH
Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.
- MeSH
- antibiotická rezistence MeSH
- monensin biosyntéza MeSH
- mutace MeSH
- propionáty farmakologie MeSH
- Streptomyces metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- monensin MeSH
- propionáty MeSH
Isoleucine added to the cultivation medium of Streptomyces cinnamonensis C-100-5 induced a relative increase of the production of monensin B at the expense of monensin A. U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of 1-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(1) of propionate. In regulatory mutants insensitive to 2-amino-3-chlorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2-12% in the presence of isoleucine which was associated with a decrease of monensin A content by 14-52%. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.
- MeSH
- aminobutyráty farmakologie MeSH
- butyráty metabolismus MeSH
- furany biosyntéza MeSH
- isoleucin metabolismus farmakologie MeSH
- monensin analogy a deriváty biosyntéza MeSH
- mutace MeSH
- norleucin farmakologie MeSH
- Streptomyces genetika metabolismus MeSH
- valin biosyntéza MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 2-amino-3-chlorobutyric acid MeSH Prohlížeč
- 2-methylbutanoic acid MeSH Prohlížeč
- aminobutyráty MeSH
- butyráty MeSH
- furany MeSH
- isoleucin MeSH
- monensin B MeSH Prohlížeč
- monensin MeSH
- norleucin MeSH
- valin MeSH
Precursors of monensins (acetate, propionate, butyrate, isobutyrate) affect the total production and the relative proportion of monensins A and B. Addition of propionate into the fermentation medium causes a prevalence of monensin B whereas butyrate and isobutyrate stimulate the production of monensin A and suppress the production of monensin B.
- MeSH
- acetáty farmakologie MeSH
- butyráty farmakologie MeSH
- furany biosyntéza MeSH
- isobutyráty MeSH
- kyselina máselná MeSH
- kyselina octová MeSH
- monensin biosyntéza MeSH
- propionáty farmakologie MeSH
- Streptomyces metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- acetáty MeSH
- butyráty MeSH
- furany MeSH
- isobutyráty MeSH
- isobutyric acid MeSH Prohlížeč
- kyselina máselná MeSH
- kyselina octová MeSH
- monensin MeSH
- propionáty MeSH
- propionic acid MeSH Prohlížeč
Using the gas chromatography-mass spectrometry (GC-MS) method we found in Streptomyces cinnamonensis saturated fatty acids of iso- and anteiso- types, cyclopropyl acids, and unsaturated fatty acids where the double bond position was determined by MS of their pyrrolidine derivatives.
Substances studied at this department in 1954-1983 are reviewed; a total of 226 compounds are characterized in a tabular form. They include natural compounds as well as those prepared by biotransformation, by semisynthetic and synthetic methods.
- MeSH
- antibakteriální látky izolace a purifikace MeSH
- mikrobiologie MeSH
- organická chemie * MeSH
- pojmy organické chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
