Serotyping and some potential virulence-associated markers were investigated in Plesiomonas shigelloides strains isolated from humans, animals and aquatic environments. Surface properties of these strains were evaluated using Congo red binding, salt-aggregation test, bacterial adherence to xylene and motility. Production of pancreatic elastase, proteinase (consistent with subtilisin Carlsberg), triacylglycerol lipase, histidine decarboxylase and beta-hemolysin was also determined. In addition, detection of signal molecules such as C4-C8 unsubstituted N-acylhomoserine lactones (AHLs) was performed. The serological typing of the P. shigelloides strains showed that the isolates belonged to 13 different serovars. The majority of the strains were hydrophobic and motile. The strains produced low levels of elastase, proteinase and histidine decarboxylase whereas triacylglycerol lipase activity was relatively high. Only 23.3 % of the strains produced hemolysin. The AHLs signal molecules were not detected. P. shigelloides strains were able to produce a variety of potential virulence markers which may be involved in the pathogenesis of Plesiomonas-associated infections.

• MeSH
• bakteriální adheze MeSH
• druhová specificita MeSH
• gama-butyrolakton analogy a deriváty   metabolismus   MeSH
• gramnegativní bakteriální infekce mikrobiologie   MeSH
• hemolyziny biosyntéza   MeSH
• histidindekarboxylasa biosyntéza   MeSH
• lidé MeSH
• lipasa biosyntéza   MeSH
• mikrobiologie vody MeSH
• pankreatická elastasa biosyntéza   MeSH
• Plesiomonas klasifikace   izolace a purifikace   metabolismus   patogenita   MeSH
• sérotypizace MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• gama-butyrolakton MeSH
• hemolyziny MeSH
• histidindekarboxylasa MeSH
• homoserine lactone MeSH Prohlížeč
• lipasa MeSH
• pankreatická elastasa MeSH

Resistance to 13 antimicrobial agents, resistance to the bactericidal activity of human serum, hydrophobic properties, lipolytic activity and production of histamine were determined in a total of 50 clinical Acinetobacter spp. strains (A. baumannii, A. lwoffii, A. calcoaceticus, A. haemolyticus). None of the tested isolates showed resistance to meropenem and none of A. lwoffii, A. calcoaceticus and A. haemolyticus strains were resistant to amikacin. Forty-six strains (92%) manifested resistance to ampicillin, 90% to cefuroxime, 68% to ciprofloxacin, 58% to piperacillin, gentamicin and cotrimaxazole, 50% to cefotaxime, 44% to amikacin, 42% to ceftazidime, 38% to piperacillin/tazobactam, 24% to netilmicin and 16% to ampicillin/sulbactam. In particular, A. baumannii and A. calcoaceticus strains showed considerable antibiotic resistance. Thirty-one isolates (62%) showed serum resistance; intermediate sensitivity was found in 19 isolates (38%). The majority of the strains (72%) demonstrated a strongly hydrophobic character; 16% of isolates exhibited moderate hydrophobic properties. All strains showed lipolytic activity; production of histamine was detected in 14 of 43 strains examined.

• MeSH
• Acinetobacter klasifikace   účinky léků   metabolismus   fyziologie   MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální adheze * MeSH
• bakteriální léková rezistence MeSH
• baktericidní aktivita krve * MeSH
• histamin metabolismus   MeSH
• hydrofobní a hydrofilní interakce MeSH
• infekce bakteriemi rodu Acinetobacter mikrobiologie   MeSH
• lidé MeSH
• lipasa metabolismus   MeSH
• mikrobiální testy citlivosti MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• histamin MeSH
• lipasa MeSH