Endo- as well as exogenous adenosine exhibits anticonvulsant action. Participation of individual types of adenosine receptors was studied in present experiments in immature rats. Cortical epileptic afterdischarges were used as a model in rat pups 12, 18 and 25 days old. CCPA, an agonist of A1 adenosine receptors, decreased markedly duration of afterdischarges whereas DPCPX, an antagonist of A1 receptors, exhibited strong proconvulsant action. Action of either drug was best expressed in 12-day-old rats and it decreased with age. Drugs influencing A2A adenosine receptors (agonist CGS21680 and antagonist ZM241385) did not exhibit systematic effects in our model. Motor phenomena accompanying cortical stimulation or epileptic afterdischarge were never influenced by any of the four drugs studied. A1 adenosine receptors are important in the model of cortical seizures, especially in the youngest group studied.

• MeSH
• akční potenciály účinky léků   fyziologie   MeSH
• analýza rozptylu MeSH
• biofyzika MeSH
• elektrická stimulace MeSH
• krysa rodu Rattus MeSH
• mozková kůra cytologie   růst a vývoj   MeSH
• novorozená zvířata MeSH
• potkani Wistar MeSH
• purinergní látky farmakologie   MeSH
• receptor adenosinový A1 metabolismus   MeSH
• receptory adenosinové A2 metabolismus   MeSH
• věkové faktory MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• purinergní látky MeSH
• receptor adenosinový A1 MeSH
• receptory adenosinové A2 MeSH

Potentiation of adenosinergic inhibitory modulation is one of possible strategies to develop new antiepileptic drugs. Nonspecific receptor agonist 2-chloroadenosine was tested against pentetrazol-induced convulsions in immature (7, 12, 18 and 25 days old) and adult rats. Doses of 1-15 mg/kg i.p. suppressed tonic phase of generalized tonic-clonic seizures (GTCS) in the two youngest groups, whereas GTCS were abolished in older rats. Minimal clonic seizures in 18-day and older rats were suppressed by high doses of 2-chloroadenosine. The role of A1 and A2A adenosine receptors was studied in 12- and 25-day-old rats. Action of an agonist of A1 receptors CCPA is similar to that of 2-chloroadenosine. An agonist of A2A receptors CGS 21680 exhibits an anticonvulsant action only in the dose-inducing catalepsy; an A2A antagonist ZM 241385 moderately suppressed tonic phase of GTCS only in 12-day-old animals. Anticonvulsant action of adenosine agonists is due to their effects on A1 receptors.

• MeSH
• 2-chloradenosin farmakologie   MeSH
• antikonvulziva farmakologie   MeSH
• konvulziva toxicita   MeSH
• krysa rodu Rattus MeSH
• pentylentetrazol toxicita   MeSH
• potkani Wistar MeSH
• receptor adenosinový A1 účinky léků   metabolismus   MeSH
• záchvaty chemicky indukované   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 2-chloradenosin MeSH
• antikonvulziva MeSH
• konvulziva MeSH
• pentylentetrazol MeSH
• receptor adenosinový A1 MeSH