Amino acids are key sources of nitrogen for growth of Candida albicans. In order to detect and take up these amino acids from a broad range of different and changing nitrogen sources inside the host, this fungus must be able to adapt via its expression of genes for amino acid uptake and further metabolism. We analyzed six C. albicans putative general amino acid permeases based on their homology to the Saccharomyces cerevisiae Gap1 general amino acid permease. We generated single- and multiple-deletion strains and found that, based on growth assays and transcriptional or posttranscriptional regulation, Gap2 is the functional orthologue to ScGap1, with broad substrate specificity. Expression analysis showed that expression of all GAP genes is under control of the Csy1 amino acid sensor, which is different from the situation in S. cerevisiae, where the expression of ScGAP1 is not regulated by Ssy1. We show that Gap4 is the functional orthologue of ScSam3, the only S-adenosylmethionine (SAM) transporter in S. cerevisiae, and we report that Gap4 is required for SAM-induced morphogenesis. IMPORTANCECandida albicans is a commensal organism that can thrive in many niches in its human host. The environmental conditions at these different niches differ quite a bit, and this fungus must be able to sense these changes and adapt its metabolism to them. Apart from glucose and other sugars, the uptake of amino acids is very important. This is underscored by the fact that the C. albicans genome encodes 6 orthologues of the Saccharomyces. cerevisiae general amino acid permease Gap1 and many other amino acid transporters. In this work, we characterize these six permeases and we show that C. albicans Gap2 is the functional orthologue of ScGap1 and that C. albicans Gap4 is an orthologue of ScSam3, an S-adenosylmethionine (SAM) transporter. Furthermore, we show that Gap4 is required for SAM-induced morphogenesis, an important virulence factor of C. albicans.

• Klíčová slova
• Candida albicans, GAP1, S-adenosyl methionine, general amino acid permease, morphogenesis,
• Publikační typ
• časopisecké články MeSH

• MeSH
• Candida albicans genetika   metabolismus   MeSH
• cykloheximid farmakologie   MeSH
• fungální proteiny genetika   MeSH
• kinetika MeSH
• koncentrace vodíkových iontů MeSH
• lysin metabolismus   MeSH
• membránové proteiny genetika   MeSH
• membránové transportní proteiny genetika   metabolismus   MeSH
• proteiny Munc18 MeSH
• proteiny nervové tkáně genetika   MeSH
• proteiny z Escherichia coli * MeSH
• regulace genové exprese u hub * MeSH
• Saccharomyces cerevisiae - proteiny * MeSH
• Saccharomyces cerevisiae genetika   metabolismus   MeSH
• transportní systémy aminokyselin MeSH
• transportní systémy pro bazické aminokyseliny * MeSH
• vezikulární transportní proteiny * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• CAN1 protein, Candida albicans MeSH Prohlížeč
• cykloheximid MeSH
• fungální proteiny MeSH
• LYP1 protein, S cerevisiae MeSH Prohlížeč
• lysin MeSH
• lysP protein, E coli MeSH Prohlížeč
• membránové proteiny MeSH
• membránové transportní proteiny MeSH
• proteiny Munc18 MeSH
• proteiny nervové tkáně MeSH
• proteiny z Escherichia coli * MeSH
• Saccharomyces cerevisiae - proteiny * MeSH
• SEC1 protein, S cerevisiae MeSH Prohlížeč
• SHR3 protein, S cerevisiae MeSH Prohlížeč
• transportní systémy aminokyselin MeSH
• transportní systémy pro bazické aminokyseliny * MeSH
• vezikulární transportní proteiny * MeSH

• MeSH
• aminokyseliny metabolismus   MeSH
• Candida albicans genetika   MeSH
• Escherichia coli genetika   MeSH
• fungální proteiny * MeSH
• geny hub genetika   MeSH
• membránové transportní proteiny genetika   MeSH
• molekulární sekvence - údaje MeSH
• sekvence aminokyselin MeSH
• sekvenční homologie aminokyselin MeSH
• sekvenční seřazení MeSH
• transportní systémy aminokyselin * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aminokyseliny MeSH
• CAN1 protein, Candida albicans MeSH Prohlížeč
• fungální proteiny * MeSH
• membránové transportní proteiny MeSH
• transportní systémy aminokyselin * MeSH