Antineutrophil autoantibodies reacting with cytoplasmic antigens are associated with various types of vasculitides, whereas antibodies reacting with neutrophil membrane antigens are mostly related to autoimmune neutropenias. The aim of this study was the investigation of the effect of monoclonal antibodies (MoAbs) reacting with surface and cytoplasmic antigens of polymorphonuclear leukocytes (PMN) known to be targets for autoantibodies in human diseases. Blood of healthy volunteers was tested for several phagocytic functions in the presence of MoAbs against surface (CD16, CD11b, CD18, NB1) and cytoplasmic (proteinase 3; PR3) molecules. Candidacidal activity was significantly inhibited in the presence of all MoAbs but isotypic control. Phagocytic activity was inhibited by anti-CD11b and/or anti-CD18 MoAbs. Zymosan-induced chemiluminescence was reduced by MoAbs anti-CD16, CD18, and NB1, enhanced by anti-PR3 MoAb, and less enhanced by anti-CD11b. In conclusion, antimembrane antibodies diminished phagocytic functions at multiple steps; in contrast, anticytoplasmic MoAb promoted activation of oxidative burst in addition to impairment of microbicidal activity. This fact may be related to different pathogenic aspects of diseases associated with antimembrane and anticytoplasmic antibodies.

• MeSH
• antigeny CD18 imunologie   MeSH
• antigeny povrchové imunologie   MeSH
• autoantigeny imunologie   MeSH
• autoimunita imunologie   MeSH
• autoprotilátky imunologie   metabolismus   MeSH
• Candida albicans imunologie   MeSH
• cytoplazma imunologie   metabolismus   MeSH
• fagocytóza účinky léků   imunologie   MeSH
• lidé MeSH
• luminiscenční měření MeSH
• makrofágový antigen 1 imunologie   MeSH
• monoklonální protilátky imunologie   farmakologie   MeSH
• neutrofily imunologie   metabolismus   MeSH
• reakce antigenu s protilátkou MeSH
• receptory IgG imunologie   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD18 MeSH
• antigeny povrchové MeSH
• autoantigeny MeSH
• autoprotilátky MeSH
• makrofágový antigen 1 MeSH
• monoklonální protilátky MeSH
• receptory IgG MeSH