The functional consequences of spreading depression (SD) during the evolution of ischemic damage was studied in two models: focal cortical ischemia induced by photothrombosis of the middle cerebral artery (MCA) and systemic hypoxia induced by 0.8% carbon monoxide (CO). These studies showed that cortical waves of SD, arising spontaneously during MCA thrombosis and after arterial occlusion delayed thrombus formation and promoted the establishment of a collateral blood supply in the perifocal zone of ischemic lesions. The underlying mechanism consisted of episodes of intense vasodilation at the decay phase of every wave of SD. Respiration of 0.8% CO increased the blood carboxyhemoglobin level to 50-60%. In lightly anesthetized rats (pentobarbital 20 mg/kg), cortical and subcortical spontaneous waves of SD were transformed into stable hypoxic depolarization, leading to death of 60% of the animals or severe lesions of the central nervous system, in 20% of animals. Increases in the level of anesthesia (50 mg/kg anesthetic) prevented the spontaneous appearance of SD during long-lasting exposure to CO. In these conditions, experimentally induced waves of SD demonstrated that the hippocampus has a high sensitivity to moderate levels of hypoxia. The duration of hypoxic depolarization of the hippocampus, provoking a single SD wave, reached 30-60 min. Selective neuron damage in field CA1 was seen 30 days after hypoxia. Additionally, the left hippocampus of rats frequently showed profound morphological lesions in the form of "granules." Cerebrolysine (2.5 ml/kg daily for 10 days) completely prevented the formation of these lesions.

• MeSH
• aminokyseliny terapeutické užití   MeSH
• anestezie MeSH
• arteria cerebri media patofyziologie   MeSH
• elektrody MeSH
• hipokampus patofyziologie   MeSH
• hypoxie farmakoterapie   patofyziologie   MeSH
• intrakraniální embolie a trombóza farmakoterapie   patofyziologie   MeSH
• karboxyhemoglobin metabolismus   MeSH
• krysa rodu Rattus MeSH
• membránové potenciály fyziologie   MeSH
• mozek účinky léků   patofyziologie   MeSH
• mozková hypoxie a ischemie farmakoterapie   patofyziologie   MeSH
• mozková kůra fyziologie   MeSH
• neokortex patologie   patofyziologie   MeSH
• nootropní látky terapeutické užití   MeSH
• otrava oxidem uhelnatým etiologie   patofyziologie   MeSH
• šířící se kortikální deprese fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminokyseliny MeSH
• cerebrolysin MeSH Prohlížeč
• karboxyhemoglobin MeSH
• nootropní látky MeSH