In the endeavour to develop a model for studying gene therapy of cancers associated with human papillomaviruses (HPVs), mouse cells were transformed with the HPV type 16 (HPV16) and activated H-ras oncogenes. This was done by cotransfection of plasmid p16HHMo, carrying the HPV16 E6/E7 oncogenes, and plasmid pEJ6.6, carrying the gene coding for human H-ras oncoprotein activated by the G12V mutation, into secondary C57BL/6 mouse kidney cells. An oncogenic cell line, designated MK16/1/IIIABC, was derived. The epithelial origin of the cells was confirmed by their expression of cytokeratins. No MHC class I and class II molecules were detected on the surface of MK16/1/IIIABC cells. Spontaneous metastases were observed in lymphatic nodes and lungs after prolonged growth of MK16/1/IIIABC-induced subcutaneous tumours. Lethally irradiated MK16/1/IIIABC cells induced protection against challenge with 10(5) homologous cells, but not against a higher cell dose (5 x 10(5)). Plasmids p16HHMo and pEJ6.6 were also used for preventive immunization of mice. In comparison with a control group injected with pBR322, they exhibited moderate protection, in terms of prolonged survival, against MK16/1/IIIABC challenge (P < 0.03). These data suggest that MK16/1/IIIABC cells may serve as a model for studying immune reactions against HPV16-associated human tumours.

• MeSH
• Cell Line MeSH
• Neoplasms, Experimental immunology   metabolism   pathology   MeSH
• Gene Expression MeSH
• Immunoblotting MeSH
• Immunohistochemistry MeSH
• Keratins analysis   MeSH
• Humans MeSH
• Neoplasm Metastasis pathology   MeSH
• Histocompatibility Antigens Class I metabolism   MeSH
• Histocompatibility Antigens Class II metabolism   MeSH
• Mice, Inbred C57BL MeSH
• Mice MeSH
• Tumor Cells, Cultured cytology   radiation effects   MeSH
• Oncogene Proteins, Viral genetics   MeSH
• Papillomaviridae genetics   MeSH
• Papillomavirus E7 Proteins MeSH
• Plasmids administration & dosage   genetics   immunology   MeSH
• Reverse Transcriptase Polymerase Chain Reaction MeSH
• Flow Cytometry MeSH
• ras Proteins genetics   metabolism   MeSH
• DNA, Recombinant MeSH
• Repressor Proteins * MeSH
• RNA genetics   metabolism   MeSH
• Transfection MeSH
• Cell Line, Transformed MeSH
• Neoplasm Transplantation MeSH
• Cell Transformation, Viral * MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Mice MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• E6 protein, Human papillomavirus type 16 MeSH Browser
• Keratins MeSH
• Histocompatibility Antigens Class I MeSH
• Histocompatibility Antigens Class II MeSH
• oncogene protein E7, Human papillomavirus type 16 MeSH Browser
• Oncogene Proteins, Viral MeSH
• Papillomavirus E7 Proteins MeSH
• ras Proteins MeSH
• DNA, Recombinant MeSH
• Repressor Proteins * MeSH
• RNA MeSH