Most cited article - PubMed ID 8832030
Successful HLA-identical sibling cord blood transplantation in a 6-year-old boy with leukocyte adhesion deficiency syndrome
Antineutrophil autoantibodies reacting with cytoplasmic antigens are associated with various types of vasculitides, whereas antibodies reacting with neutrophil membrane antigens are mostly related to autoimmune neutropenias. The aim of this study was the investigation of the effect of monoclonal antibodies (MoAbs) reacting with surface and cytoplasmic antigens of polymorphonuclear leukocytes (PMN) known to be targets for autoantibodies in human diseases. Blood of healthy volunteers was tested for several phagocytic functions in the presence of MoAbs against surface (CD16, CD11b, CD18, NB1) and cytoplasmic (proteinase 3; PR3) molecules. Candidacidal activity was significantly inhibited in the presence of all MoAbs but isotypic control. Phagocytic activity was inhibited by anti-CD11b and/or anti-CD18 MoAbs. Zymosan-induced chemiluminescence was reduced by MoAbs anti-CD16, CD18, and NB1, enhanced by anti-PR3 MoAb, and less enhanced by anti-CD11b. In conclusion, antimembrane antibodies diminished phagocytic functions at multiple steps; in contrast, anticytoplasmic MoAb promoted activation of oxidative burst in addition to impairment of microbicidal activity. This fact may be related to different pathogenic aspects of diseases associated with antimembrane and anticytoplasmic antibodies.
- MeSH
- CD18 Antigens immunology MeSH
- Antigens, Surface immunology MeSH
- Autoantigens immunology MeSH
- Autoimmunity immunology MeSH
- Autoantibodies immunology metabolism MeSH
- Candida albicans immunology MeSH
- Cytoplasm immunology metabolism MeSH
- Phagocytosis drug effects immunology MeSH
- Humans MeSH
- Luminescent Measurements MeSH
- Macrophage-1 Antigen immunology MeSH
- Antibodies, Monoclonal immunology pharmacology MeSH
- Neutrophils immunology metabolism MeSH
- Antigen-Antibody Reactions MeSH
- Receptors, IgG immunology MeSH
- Protein Binding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD18 Antigens MeSH
- Antigens, Surface MeSH
- Autoantigens MeSH
- Autoantibodies MeSH
- Macrophage-1 Antigen MeSH
- Antibodies, Monoclonal MeSH
- Receptors, IgG MeSH
