In the endeavour to develop a model for studying gene therapy of cancers associated with human papillomaviruses (HPVs), mouse cells were transformed with the HPV type 16 (HPV16) and activated H-ras oncogenes. This was done by cotransfection of plasmid p16HHMo, carrying the HPV16 E6/E7 oncogenes, and plasmid pEJ6.6, carrying the gene coding for human H-ras oncoprotein activated by the G12V mutation, into secondary C57BL/6 mouse kidney cells. An oncogenic cell line, designated MK16/1/IIIABC, was derived. The epithelial origin of the cells was confirmed by their expression of cytokeratins. No MHC class I and class II molecules were detected on the surface of MK16/1/IIIABC cells. Spontaneous metastases were observed in lymphatic nodes and lungs after prolonged growth of MK16/1/IIIABC-induced subcutaneous tumours. Lethally irradiated MK16/1/IIIABC cells induced protection against challenge with 10(5) homologous cells, but not against a higher cell dose (5 x 10(5)). Plasmids p16HHMo and pEJ6.6 were also used for preventive immunization of mice. In comparison with a control group injected with pBR322, they exhibited moderate protection, in terms of prolonged survival, against MK16/1/IIIABC challenge (P < 0.03). These data suggest that MK16/1/IIIABC cells may serve as a model for studying immune reactions against HPV16-associated human tumours.

• MeSH
• buněčné linie MeSH
• experimentální nádory imunologie   metabolismus   patologie   MeSH
• exprese genu MeSH
• imunoblotting MeSH
• imunohistochemie MeSH
• keratiny analýza   MeSH
• lidé MeSH
• metastázy nádorů patologie   MeSH
• MHC antigeny I. třídy metabolismus   MeSH
• MHC antigeny II. třídy metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádorové buňky kultivované cytologie   účinky záření   MeSH
• onkogenní proteiny virové genetika   MeSH
• Papillomaviridae genetika   MeSH
• Papillomavirus E7 - proteiny MeSH
• plazmidy aplikace a dávkování   genetika   imunologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• průtoková cytometrie MeSH
• Ras proteiny genetika   metabolismus   MeSH
• rekombinantní DNA MeSH
• represorové proteiny * MeSH
• RNA genetika   metabolismus   MeSH
• transfekce MeSH
• transformované buněčné linie MeSH
• transplantace nádorů MeSH
• virová transformace buněk * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• E6 protein, Human papillomavirus type 16 MeSH Prohlížeč
• keratiny MeSH
• MHC antigeny I. třídy MeSH
• MHC antigeny II. třídy MeSH
• oncogene protein E7, Human papillomavirus type 16 MeSH Prohlížeč
• onkogenní proteiny virové MeSH
• Papillomavirus E7 - proteiny MeSH
• Ras proteiny MeSH
• rekombinantní DNA MeSH
• represorové proteiny * MeSH
• RNA MeSH