Immunoelectrophoresis and its modifications were applied to analysis of a lipopolysaccharide-like component (LPS-LC) extracted from Borrelia garinii strains K24 and K48 isolated from Ixodes ricinus and Borrelia burgdorferi sensu stricto strain B31. A modification of the hot phenol-water method was used for isolation of LPS. Immunoelectrophoresis (IE) and crossed immunoelectrophoresis (CIE) of LPS-LC with polyclonal rabbit antisera revealed a pattern and properties partially similar to LPS from other Gram-negative bacteria. B. garinii LPS-LC formed in CIE a diffuse band extending from the start to the anode. Similarly, the shape and position of the band in IE did not show major differences from LPS of other Gram-negative bacteria. The LPS-LC extracted from the three genomic groups of B. burgdorferi sensu lato were found to have similar immunochemical properties irrespective of their genotype origin.

• MeSH
• Borrelia burgdorferi komplex chemie   genetika   imunologie   MeSH
• Borrelia burgdorferi chemie   imunologie   MeSH
• Borrelia chemie   imunologie   MeSH
• DNA bakterií genetika   MeSH
• druhová specificita MeSH
• imunoelektroforéza dvourozměrná MeSH
• imunoelektroforéza MeSH
• králíci MeSH
• lipopolysacharidy analýza   imunologie   MeSH
• protilátky bakteriální MeSH
• sekvence nukleotidů MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• DNA bakterií MeSH
• lipopolysacharidy MeSH
• protilátky bakteriální MeSH

Ixodes ricinus ticks were collected by random collections from western and central Slovakia during the years 1996-98. The midgut content of 240 ticks was examined by dark-field microscopy and submitted for cultivation for the presence of borrelias. Spirochetes were found in 21 unfed and host-seeking adults and nymphs (8.8%). By the analysis of restriction fragment length polymorphism (RFLP) one sample from unfed I. ricinus male from western Slovakia was identified as a triple infection of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii. The simultaneous presence of different B. burgdorferi genospecies in one midgut sample (triple infection in the tick) could be observed only after the multipart amplification of denaturated DNA and subsequent pooling of the products for further analysis.

• MeSH
• Borrelia burgdorferi klasifikace   genetika   izolace a purifikace   MeSH
• genotyp MeSH
• klíště mikrobiologie   MeSH
• polymerázová řetězová reakce MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH