Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.

• MeSH
• Campylobacter lari klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• Campylobacter klasifikace   genetika   izolace a purifikace   metabolismus   MeSH
• flagelin chemie   genetika   metabolismus   MeSH
• fylogeneze MeSH
• klonování DNA MeSH
• mlži mikrobiologie   MeSH
• molekulární sekvence - údaje MeSH
• mořská voda mikrobiologie   MeSH
• otevřené čtecí rámce MeSH
• polymerázová řetězová reakce MeSH
• rekombinantní proteiny chemie   genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• sekvenční analýza DNA metody   MeSH
• sekvenční seřazení MeSH
• ureasa metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Anglie MeSH
• Severní Irsko MeSH
• Názvy látek
• flaA protein, bacteria MeSH Prohlížeč
• flagelin MeSH
• rekombinantní proteiny MeSH
• ureasa MeSH

We aimed to clarify if the thermophilic Campylobacter lari organisms including urease-negative (UN) C. lari and urease-positive thermophilic Campylobacter (UPTC) can be differentiated at the species and/or subspecies levels by employing the full-length flaA gene and flaA short variable region (SVR) nucleotide sequence information or not. Thermophilic Campylobacter isolates (n = 45) including UN C. lari (n = 17), UPTC (n = 18), and Campylobacter jejuni (n = 10) were well discriminated at the isolate level by the unweighted pair group method using arithmetic means analysis and neighbor joining procedures constructed based on the full-length flaA gene and flaA SVR nucleotide sequence information. Thus, these procedures may possibly be useful for epidemimological studies for C. lari and C. jejuni.

• MeSH
• Campylobacter jejuni klasifikace   genetika   izolace a purifikace   MeSH
• Campylobacter lari klasifikace   genetika   izolace a purifikace   MeSH
• DNA bakterií chemie   genetika   MeSH
• flagelin genetika   MeSH
• genotyp MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• polymorfismus genetický * MeSH
• sekvenční analýza DNA MeSH
• shluková analýza MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA bakterií MeSH
• flaA protein, bacteria MeSH Prohlížeč
• flagelin MeSH

The PCR amplicons (about 1450 bp in length) of flaA gene fragments of 11 isolates of urease-positive thermophilic Campylobacter (UPTC) isolated from the natural environment not including wild birds in Northern Ireland were demonstrated to be shorter than those of C. jejuni 81116 and six isolates of C. jejuni and C. coli (about 1700 bp) isolated in Northern Ireland and Japan. When the nucleotide lengths of the possible open reading frame (ORF) of the flaA genes were determined, those from the 11 UPTC isolates were estimated to be 1464-1503 bp, and those from the six C. jejuni and C. coli isolates and C. jejuni 81116 strain to be 1716-1728 bp. Nucleotide sequence and deduced amino acid sequence alignments of the possible ORFs demonstrated that the ORFs from the 11 UPTC isolates lack about 80 amino acid residues, mainly from the approximate residue numbers 390-470 of the large variable region in the flaA protein of the seven isolates of C. jejuni and C. coli, and do not have any internal termination codons. High amino acid sequence similarity of both amino- and carboxy-termini of the ORFs of the flaA gene was demonstrated between the 11 isolates of UPTC and the 7 isolates of C. jejuni and C. coli. The 11 UPTC isolates examined were strongly suggested to possess a shorter flaA gene without any internal termination codons.

• MeSH
• Campylobacter coli genetika   izolace a purifikace   MeSH
• Campylobacter jejuni genetika   izolace a purifikace   MeSH
• flagelin genetika   MeSH
• molekulární sekvence - údaje MeSH
• otevřené čtecí rámce MeSH
• polymerázová řetězová reakce MeSH
• sekvence aminokyselin MeSH
• sekvenční analýza proteinů MeSH
• sekvenční seřazení MeSH
• ureasa metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Japonsko MeSH
• Severní Irsko MeSH
• Názvy látek
• flaA protein, bacteria MeSH Prohlížeč
• flagelin MeSH
• ureasa MeSH