The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH.

• Publikační typ
• časopisecké články MeSH

Spontaneous frequencies of stable chromosomal aberrations in farm animals have not been established yet. The aim of this study was to determine the spontaneous background frequencies of structural chromosomal aberrations in cattle and pig, and to compare them with the established findings in humans. Analysis was carried out on peripheral blood samples taken from 29 cows, 15 calves, 15 boars, 13 piglets, and 23 adult and 12 newborn humans. Dual-colored FISH using whole chromosome painting probes specific for human chromosomes 1 and 4, bovine chromosomes 1 and 7, and pig chromosomes 1 and 13 was performed. Chromosome aberrations were classified according to the PAINT nomenclature. The proportions of aberrant cells and the genomic frequencies of translocations, insertions and dicentrics were measured. The highest background translocation frequency was observed in humans (1.40 +/- 0.92). Data obtained in boars were similar to those obtained in humans. Cows showed much lower values of studied parameters than was expected. There was no statistical difference in any category of aberration frequencies between cows and calves. Significant differences in genomic frequencies of both total and reciprocal translocations were found when comparing boars with piglets and adult humans with newborn babies. Very low levels of spontaneous background translocation frequencies were seen among calves, piglets and newborn human babies.

• MeSH
• chromozomální aberace * MeSH
• DNA sondy ultrastruktura   MeSH
• genom * MeSH
• hybridizace in situ fluorescenční metody   MeSH
• lidé MeSH
• malování chromozomů MeSH
• metafáze MeSH
• prasata MeSH
• skot MeSH
• translokace genetická * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA sondy MeSH

Molecular epidemiology is a new and evolving area of research, combining laboratory measurement of internal dose, biologically effective dose, biologic effects, and influence of individual susceptibility with epidemiologic methodologies. Biomarkers evaluated were selected according to basic scheme: biomarkers of exposure--metabolites in urine, DNA adducts, protein adducts, and Comet assay parameters; biomarkers of effect--chromosomal aberrations, sister chromatid exchanges, micronuclei, mutations in the hypoxanthine-guanine phosphoribosyltransferase gene, and the activation of oncogenes coding for p53 or p21 proteins as measured on protein levels; biomarkers of susceptibility--genetic polymorphisms of genes CYP1A1, GSTM1, GSTT1, NAT2. DNA adducts measured by 32P-postlabeling are the biomarker of choice for the evaluation of exposure to polycyclic aromatic hydrocarbons. Protein adducts are useful as a biomarker for exposure to tobacco smoke (4-aminobiphenyl) or to smaller molecules such as acrylonitrile or 1,3-butadiene. Of the biomarkers of effect, the most common are cytogenetic end points. Epidemiologic studies support the use of chromosomal breakage as a relevant biomarker of cancer risk. The use of the Comet assay and methods analyzing oxidative DNA damage needs reliable validation for human biomonitoring. Until now there have not been sufficient data to interpret the relationship between genotypes, biomarkers of exposure, and biomarkers of effect for assessing the risk of human exposure to mutagens and carcinogens.

• MeSH
• karcinogeny analýza   toxicita   MeSH
• lidé MeSH
• molekulární epidemiologie * MeSH
• mutageny analýza   toxicita   MeSH
• pracovní expozice statistika a číselné údaje   MeSH
• vystavení vlivu životního prostředí statistika a číselné údaje   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• karcinogeny MeSH
• mutageny MeSH