Ribosomal ITS1 and ITS2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. Analysis of the ITS1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and this classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with the highest possible bootstrap proportion values of 100%. Sequence analysis of ITS regions is a powerful tool for classification of anaerobic fungi but morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.

• MeSH
• Rumen microbiology   MeSH
• beta-Glucosidase metabolism   MeSH
• Cellulases metabolism   MeSH
• Cellulose 1,4-beta-Cellobiosidase metabolism   MeSH
• Chytridiomycota classification   genetics   isolation & purification   MeSH
• DNA, Fungal chemistry   isolation & purification   MeSH
• Phylogeny MeSH
• Genetic Variation MeSH
• Fungi classification   cytology   genetics   isolation & purification   metabolism   MeSH
• DNA, Ribosomal Spacer chemistry   isolation & purification   MeSH
• Molecular Sequence Data MeSH
• Neocallimastigales classification   cytology   genetics   isolation & purification   metabolism   MeSH
• Sequence Analysis, DNA MeSH
• Sequence Homology MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• beta-Glucosidase MeSH
• Cellulases MeSH
• Cellulose 1,4-beta-Cellobiosidase MeSH
• DNA, Fungal MeSH
• DNA, Ribosomal Spacer MeSH