Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15227788
DOI
10.1007/bf02931392
Knihovny.cz E-zdroje
- MeSH
- bachor mikrobiologie MeSH
- beta-glukosidasa metabolismus MeSH
- celulasy metabolismus MeSH
- celulosa-1,4-beta-cellobiosidasa metabolismus MeSH
- Chytridiomycota klasifikace genetika izolace a purifikace MeSH
- DNA fungální chemie izolace a purifikace MeSH
- fylogeneze MeSH
- genetická variace MeSH
- houby klasifikace cytologie genetika izolace a purifikace metabolismus MeSH
- mezerníky ribozomální DNA chemie izolace a purifikace MeSH
- molekulární sekvence - údaje MeSH
- Neocallimastigales klasifikace cytologie genetika izolace a purifikace metabolismus MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-glukosidasa MeSH
- celulasy MeSH
- celulosa-1,4-beta-cellobiosidasa MeSH
- DNA fungální MeSH
- mezerníky ribozomální DNA MeSH
Ribosomal ITS1 and ITS2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. Analysis of the ITS1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and this classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with the highest possible bootstrap proportion values of 100%. Sequence analysis of ITS regions is a powerful tool for classification of anaerobic fungi but morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.
Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic Prague Czechia
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GENBANK
AY429666, AY429667, AY429669, AY429670, AY429671, AY429672, AY429673
