Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15227788
DOI
10.1007/bf02931392
Knihovny.cz E-resources
- MeSH
- Rumen microbiology MeSH
- beta-Glucosidase metabolism MeSH
- Cellulases metabolism MeSH
- Cellulose 1,4-beta-Cellobiosidase metabolism MeSH
- Chytridiomycota classification genetics isolation & purification MeSH
- DNA, Fungal chemistry isolation & purification MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Fungi classification cytology genetics isolation & purification metabolism MeSH
- DNA, Ribosomal Spacer chemistry isolation & purification MeSH
- Molecular Sequence Data MeSH
- Neocallimastigales classification cytology genetics isolation & purification metabolism MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-Glucosidase MeSH
- Cellulases MeSH
- Cellulose 1,4-beta-Cellobiosidase MeSH
- DNA, Fungal MeSH
- DNA, Ribosomal Spacer MeSH
Ribosomal ITS1 and ITS2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. Analysis of the ITS1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and this classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with the highest possible bootstrap proportion values of 100%. Sequence analysis of ITS regions is a powerful tool for classification of anaerobic fungi but morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.
Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic Prague Czechia
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