- MeSH
- laserová terapie * MeSH
- lidé MeSH
- zubní lékařství * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- úvodníky MeSH
OBJECTIVE: The goal of this investigation is to analyze the effect of therapeutic low-level laser therapy (LLLT) to have a possibility to check pain and inflammation connected with surgical removal of impacted lower third molars in general anesthesia or even phobia [not accompanied by pain or fear of dental treatment, using immunologic markers-secretory immunoglobulin A (sIgA) and lysozyme]. The healing process was also monitored by infrared thermography. BACKGROUND DATA: LLLT can accelerate the proliferation phase of healing and decrease the inflammatory reaction, but the effect is not really clear. MATERIALS AND METHODS: The treatment group comprised 213 impacted third molars (144 laser group and 74 placebo group). Laser radiation (diode laser 830 nm) was applied. The effectivity of laser therapy was evaluated based on immunological tests, that is, before and after treatment with sIgA and lysozyme in nonstimulated saliva. Thermographic examination was performed by infrared camera. RESULTS: After laser irradiation, the sIgA decreases from 546.91 mg/L (SD 354.58) to 304.91 mg/L (SD 191.96), and in the control group from 602.25 mg/L (SD 343.62) to 425.62 mg/L (SD 220.51); the differences were significant, the lysozyme value being lower. After laser therapy, the laser and placebo sides in the area of the third molars were differed in 0.2°C. CONCLUSIONS: The 830 nm wavelength penetrates to deep-seated tissues. A positive association was found between concentration of salivary sIgA and lysozyme in the saliva after LLLT application. The deep-seated wounds after wisdom teeth extraction had no effect on temperature rise in the face.
- Klíčová slova
- infrared thermography, low-level laser therapy, lysozyme, photobiomodulation, secretory IgA, wound healing,
- MeSH
- dospělí MeSH
- extrakce zubů * MeSH
- hojení ran účinky záření MeSH
- imunoglobulin A analýza MeSH
- laserová terapie s nízkou intenzitou světla metody MeSH
- lidé MeSH
- měření bolesti MeSH
- mladiství MeSH
- molár třetí chirurgie MeSH
- muramidasa analýza MeSH
- pooperační bolest prevence a kontrola MeSH
- sliny chemie MeSH
- termografie MeSH
- výsledek terapie MeSH
- zaklíněný zub chirurgie MeSH
- zánět prevence a kontrola MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- imunoglobulin A MeSH
- muramidasa MeSH
OBJECTIVE: The aim of the study was to evaluate the influence of Er:YAG laser irradiation on the debonding of metal and ceramic brackets and enamel damage ex vivo. BACKGROUND DATA: The principle of safe bracket debonding is to degrade the adhesive resin strength connecting the tooth and bracket. Removal of adhesive resin from tooth surfaces without iatrogenic damage (enamel loss) is generally the main problem of the otherwise very successful method of aesthetic straightening of teeth. METHODS: Forty ceramic and metal brackets (Clarity™ Advanced and Victory Series™; 3M Unitek, Monrovia, CA) were standardly bonded to buccal polished enamel surfaces of 30 caries-free human third molars. Two types of adhesive resins (Transbond™ XT Light Cure Adhesive; 3M Unitek, and Variolink II Professional Set; Ivoclar Vivadent AG) were used. Before debonding, the brackets in the laser group were irradiated with the Er:YAG laser (FJFI CVUT) 280 mJ, 250 μs long, repetition rate 6 Hz, spot focus 1 mm, and 140 sec. The control group was debonded without the laser irradiation. During the bracket irradiation, temperature changes inside the tooth were monitored using a thermal image infrared camera. The enamel surface was investigated by SEM. RESULTS: It has been observed that bracket removal was easier after the Er:YAG laser irradiation, and temperature rise was limited (from 2.0°C to 3.2°C) also for metal brackets. As against the nonirradiated samples, SEM investigation has confirmed no damage to enamel. CONCLUSIONS: Irradiation with Er:YAG laser radiation before debonding of ceramic brackets significantly decreases the bonding failure and amount of remaining adhesive.
- MeSH
- dospělí MeSH
- lasery pevnolátkové * MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- mladiství MeSH
- molár třetí MeSH
- ortodontické zámky * MeSH
- povrchové vlastnosti MeSH
- techniky in vitro MeSH
- testování materiálů MeSH
- zubní lepené konstrukce - odstranění přístrojové vybavení MeSH
- zubní sklovina zranění účinky záření MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: The aim of our study was to evaluate, from a histological point of view, the effect of photobiomodulation (PBM) with combined low-level laser therapy (LLLT)/light- emitting diode (LED) on porcine skin wound healing. BACKGROUND DATA: Most LLLT/LED wound healing studies have been performed on various types of rat models, with their inherent limitations. Minipigs are evolutionary and physiologically closer to humans than rats. MATERIALS AND METHODS: With the animals under general anesthesia, one full-thickness skin incision was performed on the back of each minipig (n = 10) and immediately closed using simple interrupted percutaneous sutures. The minipigs were randomly allocated into two groups: a PBM-treated group (LLLT λ = 685 nm, LED λ = 470 nm, both light sources producing power densities at 0.008 W/cm2; each light source delivering total daily doses of 3.36 J/cm2) and a sham-irradiated control group. Half of the animals in each group were killed on postoperative day 3, and the other half were killed on the postoperative day 7, and samples were removed for histological examination. RESULTS: Combined red and blue PBM accelerated the process of re-epithelization and formation of cross-linked collagen fibers compared with sham irradiated control wounds. CONCLUSIONS: Our results demonstrate that the current dose of combined red and blue PBM improves the healing of sutured skin incisions in minipigs.
OBJECTIVE: The purpose of this study was to investigate the radioprotective features of 940 nm laser on the life span of mice, and absolute counts of blood cells and their proportions in gamma-irradiated mice. BACKGROUND DATA: An important feature of laser light is activation of mitotic division and differentiation of cells, which may be useful in activation of hematopoiesis in gamma-irradiated organisms. MATERIALS AND METHODS: Mice were randomly assigned to 11 groups according to the type(s) of influence. Generally, mice were irradiated in three different ways: with laser at different fluences, with gamma irradiation, or by combination of laser at different fluences and gamma irradiation in a different order. Mice were treated with 940 nm laser at 3, 12, or 18 J/cm(2) and/or a lethal dose of gamma irradiation (8.7 Gy). Each group was randomly subdivided into two subgroups, in which the life span of the mice and blood cell counts (on 12th and 45th day after gamma irradiation) were analyzed. RESULTS: Laser (940 nm) at a fluence of 3 J/cm(2) significantly prolonged the life span of gamma-irradiated mice (p<0.05). In the same group, counts of white blood cells, lymphocytes, and neutrophils were higher on day 12 than in the gamma group. On day 45 after gamma irradiation, some signs of hematopoiesis repair were found in blood. There were no significant differences in counts of erythrocytes, monocytes, neutrophils, or the proportion of neutrophils between this group and the control group. CONCLUSIONS: In summary, 940 nm laser at a fluence of 3 J/cm(2) demonstrates radioprotective features in an experiment with lethally irradiated mice. Mechanisms responsible for this effect will be investigated in further studies.
- MeSH
- dlouhověkost účinky záření MeSH
- krevní obraz MeSH
- laserová terapie s nízkou intenzitou světla * MeSH
- lasery polovodičové * MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- náhodné rozdělení MeSH
- záření gama * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: Low-level laser therapy (LLLT) is a treatment method commonly used in physiotherapy for musculoskeletal disorders. The aim of this study was to monitor the function of temporomandibular joint (TMJ) and surrounding tissues and compare the objective measurements of the effect of LLLT. BACKGROUND DATA: LLLT has been considered effective in reducing pain and muscular tension; thus improving the quality of patients' lives. MATERIALS AND METHODS: TMJ function was evaluated by cephalometric tracing analysis, orthopantomogram, TMJ tomogram, and computer face-bow record. Interalveolar space between central incisors before and after therapy was measured. Patients evaluated pain on the Visual Analog Scale. LLLT was performed in five treatment sessions (energy density of 15.4 J/cm(2)) by semiconductive GaAlAs laser with an output of 280 mW, emitting radiation wavelength of 830 mm. The laser supplied a spot of ~0.2 cm(2). RESULTS: Baseline comparisons between the healthy patients and patients with low-level laser application show that TMJ pain during function is based on anatomical and function changes in TMJ areas. Significant differences were seen in the posterior and anterior face height. The results comparing healthy and impaired TMJ sagittal condyle paths showed that patients with TMJ pain during function had significantly flatter nonanatomical movement during function. After therapy, the unpleasant feeling was reduced from 27.5 to 4.16 on the pain Visual Analog Scale. The pain had reduced the ability to open the mouth from 34 to 42 mm. CONCLUSIONS: The laser therapy was effective in the improvement of the range of temporomandibular disorders (TMD) and promoted a significant reduction of pain symptoms.
- MeSH
- dospělí MeSH
- kefalometrie MeSH
- laserová terapie s nízkou intenzitou světla * MeSH
- lasery polovodičové terapeutické užití MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nemoci temporomandibulárního kloubu diagnóza radioterapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: With proper noninvasive ultrasound measurement of tumor depth, case selection for laser pre-ablation followed by PDT is possible. This combination of methods provides a less invasive approach to the treatment of BCC. BACKGROUND DATA: Basal cell carcinoma (BCC) primarily affects the face, and, therefore, radical excision is problematic because of the possibility of poor aesthetic outcomes. Photodynamic therapy (PDT) offers an advantage in aesthetic outcomes, but topical PDT is only effective for tumors with a depth up to 2 mm. MATERIALS AND METHODS: Seventy-five histologically verified BCCs from 67 patients were selected and divided into three therapeutic groups based on the tumor depth, which was determined by 20 MHz skin ultrasound. The three groups were: A/<2 mm (PDT), B/ 2-3 mm (Er:YAG laser ablation+PDT), and C/>3 mm (diode laser ablation+PDT). The treatment consisted of laser ablation (or no ablation) followed by the application of methyl-aminolevulinate (MAL) and a 3-h treatment period using an occlusive bandage. Subsequently, illumination with 630 nm (MAL-PDT) was performed. MAL-PDT was repeated 1-3 weeks after the first treatment. A clinical evaluation was performed after 6 months. RESULTS: A 100% clearance rate (CR) in the group with the deepest tumors was observed. In addition, a 94.7% CR occurred in the group with tumors 2-3 mm in depth, and an 81.2% CR was observed in the group with superficial tumors. CONCLUSIONS: With proper ultrasound case selection and laser ablation before MAL-PDT, the depth of a BCC lesion is not a limiting factor for PDT, and aesthetic outcomes are very good. Therefore, ultrasound-guided ablative laser-assisted PDT of BCC can be the method of choice, particularly in aesthetically challenging cases.
- MeSH
- bazocelulární karcinom diagnostické zobrazování patologie terapie MeSH
- dospělí MeSH
- estetika MeSH
- fotochemoterapie metody MeSH
- hodnocení rizik MeSH
- imunohistochemie MeSH
- jehlová biopsie MeSH
- kohortové studie MeSH
- kombinovaná terapie MeSH
- kyselina aminolevulová analogy a deriváty farmakologie MeSH
- laserová terapie metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory kůže diagnostické zobrazování patologie terapie MeSH
- následné studie MeSH
- obličej MeSH
- prospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ultrasonografie MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- kyselina aminolevulová MeSH
- methyl 5-aminolevulinate MeSH Prohlížeč
OBJECTIVE: Hemangioma is a mesenchymal benign tumor formed by blood vessels. Anomalies affect up to 10% of children and they are more common in females than in males. The aim of this study was to evaluate hemangioma treatment using four different types of lasers, namely, alexandrite, Er:YAG, CO(2), and pulsed dye laser (PDL). BACKGROUND DATA: The argon laser was the first to be used for dermatological patients, namely, with port wine stains (PWS) in the late 1960s and early 1970s. A variety of different lasers and light sources were useful in the treatment of vascular lesions, e.g., KTP, Nd:YAG, CO(2), PDL, and Er:YAG. METHODS: A group of 869 consecutive patients with hemangioma was retrospectively reviewed. The patients including in our study were divided into four groups according to the type of laser used: Alexandrite laser (n=85, 58 women and 20 men), CO(2) laser (n=78, 58 women and 20 men), Er:YAG laser (n=105, 87 women and 18 men), and PDL laser (n=601, 453 women and 148 men). All patients were treated in one session without anesthesia application. The ablative systems vaporized the tissues until the hemangioma was removed. The non-ablative systems used one shot, which destroyed the hemangioma blood vessels. RESULTS: For the treatment efficacy analysis, the following factors were evaluated: therapeutic effect (yes vs. no), loss of pigment (yes vs. no), and appearance of scar (yes vs. no). From results it was evident that the therapeutic effect of all the lasers except alexandrite was very high; almost 100%. In the CO(2) and the Er:YAG laser groups a high percentage of side effects was also observed. Exposure to these lasers caused loss of pigment and scar formation in many cases. The best therapeutic effect, with only minor side effects, has been achieved with the PDL laser. CONCLUSION: It was confirmed that PDL (595 nm, 1.5 ms, 7 mm, 9 - 11 J/cm(2)) had optimal effect without scars. Er:YAG or CO(2) laser radiation with specific characteristics was also useful for small superficial lesions.
- MeSH
- analýza rozptylu MeSH
- dospělí MeSH
- hemangiom radioterapie MeSH
- laserová terapie metody MeSH
- lasery barvivové MeSH
- lasery pevnolátkové MeSH
- lasery plynové MeSH
- lidé středního věku MeSH
- lidé MeSH
- neparametrická statistika MeSH
- retrospektivní studie MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: The aim of this study was to prepare a simple and reliable method for ceramic bracket debonding, ensuring minimal changes in the enamel structure and an acceptable temperature rise in the pulp. BACKGROUND DATA: Ceramic bracket debonding is based on the principle of degrading the strength of adhesive resin between the tooth and ceramic bracket. The search for a safe and efficient method of adhesive resin removal following debonding has resulted in the introduction of a wide range of instruments and procedures, among which proper use of laser irradiation can be promising. METHODS: The debonding of two types of ceramic brackets utilized a diode-pumped Thulium:Ytterbium-Aluminium-Perovskite (Tm:YAP) microchip laser generating irradiation at a wavelength of 1998 nm (spot size 3 mm; focused by lens), with two power settings (1-2 W). Loss of enamel and residual resin on teeth, as well as rise in temperature inside the tooth were subsequently investigated in detail. RESULTS: A 1W power of irradiation during a 60-sec period resulted in a temperature rise from 3 to 4°C in the approximate root location. This power is also suitable for debracketing from the point of view of damage to enamel lying below the bracket. Only a slight damage to the enamel was registered by SEM compared to conventional bracket removal. CONCLUSIONS: Use of a Tm:YAP laser (wavelength 1998 nm, power 1 W, irradiance 14 W/cm(2), interacting time 60 sec) which is at the same time compact and small enough to be used in the dental practice, together with moderate cooling, could be an efficient tool for debracketing.
- MeSH
- dítě MeSH
- hliník MeSH
- keramika chemie MeSH
- lasery polovodičové * MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- mladiství MeSH
- ortodontické zámky MeSH
- oxidy MeSH
- sloučeniny vápníku MeSH
- teplota MeSH
- thulium MeSH
- titan MeSH
- yterbium MeSH
- zubní dřeň účinky záření MeSH
- zubní lepené konstrukce - odstranění přístrojové vybavení metody MeSH
- zubní sklovina účinky záření MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hliník MeSH
- oxidy MeSH
- perovskite MeSH Prohlížeč
- sloučeniny vápníku MeSH
- thulium MeSH
- titan MeSH
- yterbium MeSH
Colorectal cancer is one of the most common cancers in Europe and North America and it is the most common gastrointestinal carcinoma. The population in the Czech Republic has a higher incidence of colorectal carcinoma compared to other countries. Efforts are underway to develop better screening strategies and novel therapies to improve patient survival rates. Despite all efforts, colorectal cancer remains one of the leading causes of death from cancer. Photodynamic therapy (PDT) is an established modality for the treatment of various diseases. The PDT procedure involves the administration of a photosensitizer followed by illumination. The anti-tumor effects result from direct killing of malignant cells, shutting down of the tumor's vasculature, and the promotion of an immune response. In our experiment, we examined the effects of phototherapy with disulfonated hydroxyaluminum phthalocyanine (Al(OH)S2Pc) on the growth of colorectal carcinoma cells, in an effort to offer a new treatment modality for patients with this disease.
- MeSH
- fotochemoterapie * MeSH
- fotosenzibilizující látky aplikace a dávkování MeSH
- kolorektální nádory farmakoterapie MeSH
- lidé MeSH
- myši nahé MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- transplantace nádorů MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fotosenzibilizující látky MeSH
