The chemoprotective effects of caffeic (CA), chlorogenic (CHA) and rosmarinic (RA) acids were tested against the toxicity of doxorubicin (DOX) in neonatal rat cardiomyocytes and the iron-dependent DOX induced lipid peroxidation of heart membranes, mitochondria and microsomes. The protectivity of these acids was compared with dexrazoxan, used as an adjuvant during DOX chemotherapy. The cytoprotective effects were assessed by enzyme (LDH and ASAT) and troponin I leakage, secondly by intracellular ATP content. All hydroxycinnamic acids proved non-cytotoxic, and they stabilized both membranes and the energetic status of cardiomyocytes. After preincubation of cardiomyocytes with the test compounds (100, 200 microm; 1 h) the cardiomyocytes were treated with the toxic agent, DOX (100 microm; 8 h). The test compounds protected cardiomyocytes against DOX induced oxidative stress (RA > CHA > or = CA) on all monitored parameters. Substantial preservation of monolayer integrity of the cardiomyocytes by test compounds was also found microscopically. All the acids were more effective in the assays used than dexrazoxan. RA showed the most effective cytoprotectivity. All the acids significantly reduced the iron-dependent DOX induced lipid peroxidation of heart membranes, although of the test compounds, CHA was found to be the most effective (IC(50) = 8.04 +/- 0.74/6.87 +/- 0.52 micro m for microsomes/mitochondria).
- MeSH
- cinnamáty aplikace a dávkování farmakologie terapeutické užití MeSH
- depsidy MeSH
- doxorubicin MeSH
- fytoterapie * MeSH
- inhibiční koncentrace 50 MeSH
- kardiomyocyty účinky léků enzymologie MeSH
- krysa rodu Rattus MeSH
- kyselina chlorogenová aplikace a dávkování farmakologie terapeutické užití MeSH
- kyselina rozmarýnová MeSH
- kyseliny kávové aplikace a dávkování farmakologie terapeutické užití MeSH
- léčivé rostliny * MeSH
- mikrozomy účinky léků MeSH
- myokard cytologie enzymologie MeSH
- nemoci srdce chemicky indukované patologie prevence a kontrola MeSH
- ochranné látky aplikace a dávkování farmakologie terapeutické užití MeSH
- peroxidace lipidů účinky léků MeSH
- potkani Wistar MeSH
- rostlinné extrakty aplikace a dávkování farmakologie terapeutické užití MeSH
- srdeční mitochondrie účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cinnamáty MeSH
- depsidy MeSH
- doxorubicin MeSH
- kyselina chlorogenová MeSH
- kyseliny kávové MeSH
- ochranné látky MeSH
- rostlinné extrakty MeSH
