Purpose: Prostate-specific antigen (PSA) is a sensitive but unspecific marker for prostate cancer (PC) detection, which may result in harms including overdiagnosis and overtreatment. Therefore, the development of new markers is of absolute value. The urinary level of engrailed-2 (EN2) protein has been recently suggested as a promising PC biomarker, correlating with tumour volume and stage. This study evaluated EN2 and its potential use in clinical practice.Materials and methods: Urinary EN2 was assessed by different commercially available enzyme-linked immunosorbent assay kits. The study sample included 90 patients with clinically localized PC compared to 30 healthy controls, and a group of 40 patients indicated for prostate biopsy due to an elevated PSA level where both pre- and post-digital rectal examination urine samples were collected.Results: No statistical difference between the patient group and the control group was obtained in all measured variables. There was no significant correlation between urinary EN2 and serum PSA, tumour staging and grading. Attentive DRE did not lead to significant changes of urinary EN2 or impact on its predictive power.Conclusions: Our results show that EN2 as a PC biomarker brings no additional value to the current use of PSA in clinical practice.

• Klíčová slova
• EN2, Prostate cancer, biomarkers, engrailed-2, urinary,
• MeSH
• analýza moči MeSH
• biopsie MeSH
• dospělí MeSH
• ELISA MeSH
• homeodoménové proteiny moč   MeSH
• kalikreiny krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové biomarkery krev   moč   MeSH
• nádory prostaty krev   diagnóza   patologie   moč   MeSH
• prediktivní hodnota testů MeSH
• prostatický specifický antigen krev   MeSH
• proteiny nervové tkáně moč   MeSH
• senioři MeSH
• staging nádorů MeSH
• studie případů a kontrol MeSH
• stupeň nádoru MeSH
• tumor burden MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• engrailed 2 protein MeSH Prohlížeč
• homeodoménové proteiny MeSH
• kalikreiny MeSH
• KLK3 protein, human MeSH Prohlížeč
• nádorové biomarkery MeSH
• prostatický specifický antigen MeSH
• proteiny nervové tkáně MeSH