Studies on Huntington's disease (HD) demonstrated altered immune response in HD gene carriers. Using multiplexing immunoassay, we simultaneously investigated seven cytokines in secretomes of microglia and blood monocytes, cerebrospinal fluid (CSF) and serum collected from transgenic HD minipigs at pre-symptomatic disease stage. Decline in IFNα and IL-10 was observed in CSF and secretome of microglia whilst elevated IL-8 and IL-1β levels were secreted by microglia. Additionally, IL-8 was increased in serum. The proportion of mutant huntingtin in microglia may have causative impact on cytokine production. IFNα, IL-10, IL-8 and IL-1β represent promising biomarkers reflecting immuno-pathological mechanisms in porcine HD model.

• Klíčová slova
• Biomarker(s), Central nervous system, Cytokines, Immune response, Porcine Huntington's disease model, Serum,
• MeSH
• biologické markery metabolismus   MeSH
• centrální nervový systém cytologie   patologie   MeSH
• cytokiny metabolismus   MeSH
• DNA vazebné proteiny metabolismus   MeSH
• geneticky modifikovaná zvířata MeSH
• Huntingtonova nemoc genetika   metabolismus   patologie   MeSH
• interferon alfa metabolismus   MeSH
• interleukin-10 metabolismus   MeSH
• interleukin-1beta metabolismus   MeSH
• interleukin-8 metabolismus   MeSH
• kultivované buňky MeSH
• lidé MeSH
• lipopolysacharidy farmakologie   MeSH
• mikrofilamentové proteiny MeSH
• mikroglie účinky léků   metabolismus   MeSH
• miniaturní prasata MeSH
• modely nemocí na zvířatech MeSH
• monocyty účinky léků   metabolismus   MeSH
• prasata MeSH
• protein huntingtin MeSH
• proteiny nervové tkáně genetika   metabolismus   MeSH
• proteiny vázající vápník MeSH
• regulace genové exprese genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• AIF1 protein, human MeSH Prohlížeč
• biologické markery MeSH
• cytokiny MeSH
• DNA vazebné proteiny MeSH
• HTT protein, human MeSH Prohlížeč
• interferon alfa MeSH
• interleukin-10 MeSH
• interleukin-1beta MeSH
• interleukin-8 MeSH
• lipopolysacharidy MeSH
• mikrofilamentové proteiny MeSH
• protein huntingtin MeSH
• proteiny nervové tkáně MeSH
• proteiny vázající vápník MeSH

Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP(®) bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody(®) microarrays (RayBiotech) and xMAP(®) assays (Luminex and its partners).

• MeSH
• buněčné kultury MeSH
• čipová analýza proteinů metody   MeSH
• cytokiny metabolismus   MeSH
• imunoanalýza metody   MeSH
• kmenové buňky fyziologie   MeSH
• kultivační média speciální metabolismus   MeSH
• kultivované buňky MeSH
• mezibuněčná komunikace * MeSH
• mezibuněčné signální peptidy a proteiny metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytokiny MeSH
• kultivační média speciální MeSH
• mezibuněčné signální peptidy a proteiny MeSH