Multiplex immunoassays for quantification of cytokines, growth factors, and other proteins in stem cell communication
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25063502
DOI
10.1007/7651_2014_94
Knihovny.cz E-resources
- MeSH
- Cell Culture Techniques MeSH
- Protein Array Analysis methods MeSH
- Cytokines metabolism MeSH
- Immunoassay methods MeSH
- Stem Cells physiology MeSH
- Culture Media, Conditioned metabolism MeSH
- Cells, Cultured MeSH
- Cell Communication * MeSH
- Intercellular Signaling Peptides and Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines MeSH
- Culture Media, Conditioned MeSH
- Intercellular Signaling Peptides and Proteins MeSH
Immunoassays represent valuable and broadly used techniques for detection and quantification of proteins. Thanks to their high sensitivity, such techniques are powerful for analyzing growth factors, trophic factors, angiogenic factors, hormones, cytokines, chemokines, soluble receptors, and other proteins which play key roles in intercellular communication and operate as potent regulators of stem cell survival, proliferation, differentiation, or cell death. Multiplex immunological assays, in contrast to ELISA, offer simultaneous quantification of tens of proteins across multiple samples, and have been developed to save time, costs, and sample volumes. Among them, planar antibody microarrays and xMAP(®) bead-based assays have become particularly popular for characterization of proteins secreted by stem cells, as they are relatively easy, highly accurate, multiplex to a high degree and a broad spectrum of analytes can be measured. Here, we describe protocols for multiplex quantification of secreted proteins using Quantibody(®) microarrays (RayBiotech) and xMAP(®) assays (Luminex and its partners).
References provided by Crossref.org
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