The concentrations of ROS were measured in samples of the sensorimotor brain cortex and in the rat blood. We measured the following parameters: The six lines spectra, nitroxide radical, free hydroxyl radical and singleton oxygen. Their concentration was measured under physiological conditions, after the nociceptive stimulation and after the application of melatonin, both in normal and stimulated animals. In the brain cortex only the singleton oxygen decreased after the nociceptive stimulation, whereas the nitroxide radicals and six lines spectra increased. The free hydroxyl radicals did not change significantly. In the blood serum the six lines spectra and nitroxide radical increased, the concentration of the free hydroxyl radicals did not change. Melatonin increased both the hydroxyl and nitroxide radicals. There was a non-significant decrease in the six lines spectra. The estimation of ROS can be used as a tool for detecting metabolic changes and the consequences of different environmental influences, in our case the influence of nociception and melatonin.
- MeSH
- analýza rozptylu MeSH
- bolest metabolismus MeSH
- hydroxylový radikál krev MeSH
- krysa rodu Rattus MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- melatonin fyziologie MeSH
- oxidy dusíku krev MeSH
- potkani Wistar MeSH
- reaktivní formy kyslíku analýza krev MeSH
- sérum chemie MeSH
- singletový kyslík krev MeSH
- somatosenzorické korové centrum chemie metabolismus MeSH
- volné radikály analýza krev MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- hydroxylový radikál MeSH
- látky reagující s kyselinou thiobarbiturovou MeSH
- melatonin MeSH
- nitroxyl MeSH Prohlížeč
- oxidy dusíku MeSH
- reaktivní formy kyslíku MeSH
- singletový kyslík MeSH
- volné radikály MeSH
