While there is substantial research on what people want in their romantic and sexual partners, much of this work focuses on WEIRD, youthful samples, fails to consider the role of undesirable characteristics (i.e., things people do not want in partners) at all, or in conjunction with desirable characteristics (i.e., things people do want in partners), and may be overly reliant on psychometric approaches to pivotal variables in mating psychology like mate value and sociosexuality. In a nationally representative (online) sample of 2280 people from Czechia (aged between 18 and 50 years old), we examined linear and quadratic age, education, and self-perceived mate value (desirability) effects on the desired levels in mate choice of eight undesirable and seven desirable characteristics in men and women in relation to ostensible metrics of mate value. Self-perceived mate value alone explained little variance (men 1%, women 2%), while all mate value and mating strategy indicators together explained little variance of mate preferences and aversions (men 3%, women 5%). Desirable characteristics were better explained by mate value than undesirable ones. Our results are in line with evolutionary predictions suggesting that women are more demanding. Also, more qualities to offer correlate with more expectations in a partner.

• Klíčová slova
• Desirability, Mate preferences, Mate value, Mating strategy, Sex differences, Undesirability,
• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• manželství * psychologie   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• sebepojetí MeSH
• sexuální chování * psychologie   MeSH
• sexuální partneři * psychologie   MeSH
• výběrové chování * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

Research shows that male body odor plays an important role in women's mate choice and that olfactory abilities are associated with women's sexual functioning. What remains unclear is what types of partner's odor actually shape women's experience during intimate activities. This study therefore explored women's experience associated with the partner's various odors and investigated how they affect women's intimate and sexual encounters. We performed semi-structured individual interviews with 20 single women and 20 women in a long-term relationship. Thematic analysis revealed four key natural odor types of the partner: body odor, sweat, genital odor, and semen odor. Further, we have identified three main types of fragrance odor (cologne, shower gel, and laundry agents) and investigated their perception in both intimate (hugging, kissing, cuddling, lying side by side) and sexual (intercourse, oral sex, ejaculation) contexts. Both partner's natural odor and fragrance affected women's emotional state (ranging from pleasant to unpleasant) and behavioral response (ranging from approach to avoidance of partner). Women's odor perception was frequently context-dependent, so that even mostly negatively perceived odors (e.g., semen, genital odor) were often accepted as part of sexual encounter. Finally, women's perception was negatively modified by partner's specific sweat (after workday, workout, or when the partner is ill) during intimate encounters. Our results highlight the complexity and interindividual variability of partner's odor perception.

• Klíčová slova
• Body odor, Chemical communication, Fragrance, Olfaction, Romantic relationship, Sex,
• MeSH
• čich fyziologie   MeSH
• čichová percepce fyziologie   MeSH
• dospělí MeSH
• interpersonální vztahy MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• odoranty * MeSH
• pot MeSH
• sexuální chování * psychologie   MeSH
• sexuální partneři * psychologie   MeSH
• sperma MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The binding of mRNAs to ribosomes is mediated by the protein complex eIF4F in conjunction with eIF4B (eukaryotic initiation factor 4F and 4B). EIF4F is a three subunit complex consisting of eIF4A (RNA helicase), eIF4E (mRNA cap binding protein), and eIF4G (bridging protein). The crucial role is played by eIF4E, which directly binds the 5'-cap structure of the mRNA and facilitates the recruitment to the mRNA of other translation factors and the 40S ribosomal subunit. EIF4E binding to mRNA and to other initiation factors is regulated on several levels, including its phosphorylation on Ser-209, and association with its regulatory protein 4E-binding protein (4E-BP1). In this study we document that both the translation initiation factor eIF4E and its regulator 4E-BP1 become dephosphorylated in the early stage porcine zygotes already 8 hr post-activation. Similarly, the activities of ERK1/2 MAP and Mnk1 kinases, which are both involved in eIF4E phosphorylation, gradually decrease during this period with the timing similar to that of eIF4E dephosphorylation. The formation of an active eIF4F complex is also diminished after 9-15 hr post-activation, although substantial amounts of this complex have been detected also 24 hr post-activation (2-cell stage). The overall protein synthesis in the parthenotes decreases gradually from 12 hr post-activation reaching a minimum after 48 hr (4-cell stage). Although the translation is gradually decreasing during early preimplantation development, the eIF4F complex, which is temporarily formed, might be a premise for the translation of a small subset of mRNAs at this period of development.

• MeSH
• časové faktory MeSH
• embryo savčí cytologie   metabolismus   MeSH
• eukaryotické iniciační faktory metabolismus   MeSH
• eukaryotický iniciační faktor 4F metabolismus   MeSH
• kultivované buňky MeSH
• mitogenem aktivovaná proteinkinasa 1 metabolismus   MeSH
• mitogenem aktivovaná proteinkinasa 3 metabolismus   MeSH
• partenogeneze fyziologie   MeSH
• prasata MeSH
• proteosyntéza fyziologie   MeSH
• ribozomy metabolismus   MeSH
• RNA čepičky metabolismus   MeSH
• transportní proteiny metabolismus   MeSH
• zvířata MeSH
• zygota cytologie   metabolismus   MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• eIF-4B MeSH Prohlížeč
• eukaryotické iniciační faktory MeSH
• eukaryotický iniciační faktor 4F MeSH
• mitogenem aktivovaná proteinkinasa 1 MeSH
• mitogenem aktivovaná proteinkinasa 3 MeSH
• RNA čepičky MeSH
• transportní proteiny MeSH

Eukaryotic initiation factor 4E (eIF4E) plays an important role in mRNA translation by binding the 5'-cap structure of the mRNA and facilitating the recruitment to the mRNA of other translation factors and the 40S ribosomal subunit. eIF4E undergoes regulated phosphorylation on Ser-209 and this phosphorylation is believed to be important for its binding to mRNA and to other initiation factors. The findings showing that the translation initiation factor eIF4E becomes gradually phosphorylated during in vitro maturation (IVM) of pig oocytes with a maximum in metaphase II (M II) stage oocytes have been documented by us recently (Ellederova et al., 2006). The aim of this work was to study in details the metabolic pathways involved in this process. Using inhibitors of cyclin-dependent kinases, Butyrolactone I (BL I) and protein phosphatases, okadaic acid (OA) we show that ERK1/2 MAP kinase pathway is involved in this phosphorylation. We also demonstrate that activation and phosphorylation of ERK1/2 MAP kinase and eIF4E is associated with the activating phosphorylation of Mnk1 kinase, one of the two main kinases phosphorylating eIF4E in somatic cells.

• MeSH
• eukaryotický iniciační faktor 4E genetika   metabolismus   MeSH
• fosforylace MeSH
• inhibitory enzymů farmakologie   MeSH
• isoelektrická fokusace MeSH
• mitogenem aktivovaná proteinkinasa 3 metabolismus   MeSH
• oocyty účinky léků   fyziologie   MeSH
• prasata MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• eukaryotický iniciační faktor 4E MeSH
• inhibitory enzymů MeSH
• mitogenem aktivovaná proteinkinasa 3 MeSH

In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis of many of these proteins was significantly decreased, we found four proteins with increased biosynthetic rate, which are supposed to play an essential role in meiosis. Among them, the ubiquitin C-terminal hydrolase-L1 (UCH-L1) was identified by mass spectrometry. To study the regulatory role of UCH-L1 in the process of meiosis in pig model, we used a specific inhibitor of this enzyme, marked C30, belonging to the class of isatin O-acyl oximes. When germinal vesicle (GV) stage cumulus-enclosed oocytes were treated with C30, GV breakdown was inhibited after 28 h of culture, and most of the oocytes were arrested at the first meiosis after 44 h. The block of metaphase I-anaphase transition was not completely reversible. In addition, the inhibition of UCH-L1 resulted in elevated histone H1 kinase activity, corresponding to cyclin-dependent kinase(CDK1)-cyclin B1 complex, and a low level of monoubiquitin. These results supported the hypothesis that UCH-L1 might play a role in metaphase I-anaphase transition by regulating ubiquitin-dependent proteasome mechanisms. In summary, a proteomic approach coupled with protein verification study revealed an essential role of UCH-L1 in the completion of the first meiosis and its transition to anaphase.

• MeSH
• 2D gelová elektroforéza MeSH
• encefalitogenní základní proteiny analýza   metabolismus   MeSH
• fertilizace in vitro MeSH
• imunoblotting MeSH
• indoly farmakologie   MeSH
• meióza účinky léků   fyziologie   MeSH
• oocyty chemie   metabolismus   MeSH
• oximy farmakologie   MeSH
• prasata MeSH
• proteasomový endopeptidasový komplex MeSH
• proteinkinasa CDC2 analýza   metabolismus   MeSH
• proteinkinasy analýza   metabolismus   MeSH
• spektrální analýza MeSH
• thiolesterasa ubikvitinu analýza   antagonisté a inhibitory   metabolismus   MeSH
• ubikvitin metabolismus   MeSH
• vaječné proteiny analýza   biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• encefalitogenní základní proteiny MeSH
• histone H1 kinase MeSH Prohlížeč
• indoly MeSH
• LDN 57444 MeSH Prohlížeč
• oximy MeSH
• proteasomový endopeptidasový komplex MeSH
• proteinkinasa CDC2 MeSH
• proteinkinasy MeSH
• thiolesterasa ubikvitinu MeSH
• ubikvitin MeSH
• vaječné proteiny MeSH

Aurora kinase B (AURKB) is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we examine expression and phosphorylation on Thr-232 of AURKB during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, whereas Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 microM butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10 microg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germinal vesicle stage, when nuclear membrane remains intact; however, condensed chromosome fibers or highly condensed chromosome bivalents can be seen in the nucleoplasm of BL-I- or cycloheximide-treated oocytes, respectively. In these treated oocytes, no or only very weak AURKB activity and phosphorylation of histone H3 on Ser-10 can be detected after 27 h of treatment, whereas phosphorylation on Ser-28 is not influenced. These results suggest that AURKB activity and Ser-10 phosphorylation of histone H3 are not required for chromosome condensation in pig oocytes, but might be required for further processing of chromosomes during meiosis.

• MeSH
• benzamidy farmakologie   MeSH
• chinazoliny farmakologie   MeSH
• chromozomy účinky léků   fyziologie   MeSH
• cykloheximid farmakologie   MeSH
• fosforylace účinky léků   MeSH
• gama-butyrolakton analogy a deriváty   farmakologie   MeSH
• histony metabolismus   MeSH
• kinasy Aurora MeSH
• meióza fyziologie   MeSH
• oocyty růst a vývoj   MeSH
• prasata MeSH
• protein-serin-threoninkinasy antagonisté a inhibitory   metabolismus   MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 4-(4-(N-benzoylamino)anilino)-6-methoxy-7-(3-(1-morpholino)propoxy)quinazoline MeSH Prohlížeč
• benzamidy MeSH
• butyrolactone I MeSH Prohlížeč
• chinazoliny MeSH
• cykloheximid MeSH
• gama-butyrolakton MeSH
• histony MeSH
• kinasy Aurora MeSH
• protein-serin-threoninkinasy MeSH