• MeSH
• dědičné nádorové syndromy patologie   MeSH
• dítě MeSH
• dospělí MeSH
• imunohistochemie MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nádorové biomarkery analýza   MeSH
• nádorové supresorové proteiny analýza   MeSH
• nádory kůže chemie   patologie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• thiolesterasa ubikvitinu analýza   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• dopisy MeSH
• Názvy látek
• BAP1 protein, human MeSH Prohlížeč
• nádorové biomarkery MeSH
• nádorové supresorové proteiny MeSH
• thiolesterasa ubikvitinu MeSH

In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis of many of these proteins was significantly decreased, we found four proteins with increased biosynthetic rate, which are supposed to play an essential role in meiosis. Among them, the ubiquitin C-terminal hydrolase-L1 (UCH-L1) was identified by mass spectrometry. To study the regulatory role of UCH-L1 in the process of meiosis in pig model, we used a specific inhibitor of this enzyme, marked C30, belonging to the class of isatin O-acyl oximes. When germinal vesicle (GV) stage cumulus-enclosed oocytes were treated with C30, GV breakdown was inhibited after 28 h of culture, and most of the oocytes were arrested at the first meiosis after 44 h. The block of metaphase I-anaphase transition was not completely reversible. In addition, the inhibition of UCH-L1 resulted in elevated histone H1 kinase activity, corresponding to cyclin-dependent kinase(CDK1)-cyclin B1 complex, and a low level of monoubiquitin. These results supported the hypothesis that UCH-L1 might play a role in metaphase I-anaphase transition by regulating ubiquitin-dependent proteasome mechanisms. In summary, a proteomic approach coupled with protein verification study revealed an essential role of UCH-L1 in the completion of the first meiosis and its transition to anaphase.

• MeSH
• 2D gelová elektroforéza MeSH
• encefalitogenní základní proteiny analýza   metabolismus   MeSH
• fertilizace in vitro MeSH
• imunoblotting MeSH
• indoly farmakologie   MeSH
• meióza účinky léků   fyziologie   MeSH
• oocyty chemie   metabolismus   MeSH
• oximy farmakologie   MeSH
• prasata MeSH
• proteasomový endopeptidasový komplex MeSH
• proteinkinasa CDC2 analýza   metabolismus   MeSH
• proteinkinasy analýza   metabolismus   MeSH
• spektrální analýza MeSH
• thiolesterasa ubikvitinu analýza   antagonisté a inhibitory   metabolismus   MeSH
• ubikvitin metabolismus   MeSH
• vaječné proteiny analýza   biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• encefalitogenní základní proteiny MeSH
• histone H1 kinase MeSH Prohlížeč
• indoly MeSH
• LDN 57444 MeSH Prohlížeč
• oximy MeSH
• proteasomový endopeptidasový komplex MeSH
• proteinkinasa CDC2 MeSH
• proteinkinasy MeSH
• thiolesterasa ubikvitinu MeSH
• ubikvitin MeSH
• vaječné proteiny MeSH