Fibroblastoid mouse L-cells are widely used in immunological models because when transfected with class II-coding genes they become efficient antigen presenting cells. Little is known, however, about the cell surface markers borne by L-cells and their putative involvement/Interference with the experimental models studied. Rats were immunized against DAP.3 cells (subclone of L-cells) and monoclonal antibodies (mAbs) were prepared. One of them, 4D4, was studied in detail. It recognizes an epitope which is neither cell lineage- nor strain- nor species-restricted since, in addition to DAP.3 cells, it binds, as determined by flow cytometry and immunohistochemistry, to various cells such as CD8+ T cells from thymus, spleen, lymph node or intestinal epithelium, mouse peritoneal B cells and various tissues such as renal, pulmonary or intestinal epithelia. 4D4 mAb immunoprecipitates an undescribed 68 kDa protein. Functionally, this mAb inhibits the IL-2 secretion of a T cell clone in response to its peptide presented by appropriate class II-transfected L-cells and induces a negative selection of double positive CD4+CD8+ thymocytes. Since the 4D4 ligand is found on cells which are submitted to selection (T cells) and on cells which mediate selection (epithelial and antigen presenting cells), we conclude that 4D4 mAb defines a cell surface antigen involved, as an accessory molecule, in a cell selection process.

• MeSH
• antigeny povrchové analýza   imunologie   MeSH
• epitopy imunologie   MeSH
• inbrední kmeny potkanů MeSH
• interleukin-2 metabolismus   MeSH
• křížení genetické MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• L buňky (buněčná linie) imunologie   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• monoklonální protilátky imunologie   MeSH
• myši inbrední BALB C MeSH
• myši inbrední C3H MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádorové buňky kultivované MeSH
• norek MeSH
• orgánová specificita MeSH
• podskupiny lymfocytů imunologie   MeSH
• potkani inbrední BN MeSH
• prezentace antigenu * MeSH
• specificita protilátek MeSH
• transfekce MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH
• Názvy látek
• 4D4 antigen MeSH Prohlížeč
• antigeny povrchové MeSH
• epitopy MeSH
• interleukin-2 MeSH
• monoklonální protilátky MeSH