A prelabor rupture of membranes (PROM) and its subtypes, preterm PROM (pPROM) and term PROM (tPROM), are associated with disturbances in the hemostatic system and angiogenesis. This study was designed to demonstrate the role of single nucleotide polymorphisms (SNPs), localized in CSF2 (rs25881), FLT1 (rs722503), TFPI (C-399T) and TLR9 (rs352140) genes, in PROM. A population of 360 women with singleton pregnancy consisted of 180 PROM cases and 180 healthy controls. A single-SNP analysis showed a similar distribution of genotypes in the studied polymorphisms between the PROM or the pPROM women and the healthy controls. Double-SNP TT variants for CSF2 and FLT1 polymorphisms, CC variants for TLR9 and TFPI SNPs, TTC for CSF2, FLT1 and TLR9 polymorphisms, TTT for FLT1, TLR9 and TFPI SNPs and CCCC and TTTC complex variants for all tested SNPs correlated with an increased risk of PROM after adjusting for APTT, PLT parameters and/or pregnancy disorders. The TCT variants for the CSF2, FLT1 and TLR9 SNPs and the CCTC for the CSF2, FLT1, TLR9 and TFPI polymorphisms correlated with a reduced risk of PROM when corrected by PLT and APTT, respectively. We concluded that the polymorphisms of genes, involved in hemostasis and angiogenesis, contributed to PROM.

• Klíčová slova
• angiogenesis, genotyping, hemostasis, pPROM, pregnancy, prelabor rupture of membranes (PROM), restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP), tPROM,
• MeSH
• dospělí MeSH
• faktor stimulující granulocyto-makrofágové kolonie genetika   MeSH
• genetická predispozice k nemoci MeSH
• genetické asociační studie MeSH
• jednonukleotidový polymorfismus * MeSH
• lidé MeSH
• lipoproteiny genetika   MeSH
• mladý dospělý MeSH
• předčasný odtok plodové vody genetika   MeSH
• receptor 1 pro vaskulární endoteliální růstový faktor genetika   MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• toll-like receptor 9 genetika   MeSH
• věk matky MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• CSF2 protein, human MeSH Prohlížeč
• faktor stimulující granulocyto-makrofágové kolonie MeSH
• FLT1 protein, human MeSH Prohlížeč
• lipoprotein-associated coagulation inhibitor MeSH Prohlížeč
• lipoproteiny MeSH
• receptor 1 pro vaskulární endoteliální růstový faktor MeSH
• TLR9 protein, human MeSH Prohlížeč
• toll-like receptor 9 MeSH

The transcription factor c-MYB is a well-known marker of undifferentiated cells such as haematopoietic cell precursors, but recently it has also been observed in differentiated cells that produce hard tissues. Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in c-myb-overexpressing cultures, whereas downregulation of c-myb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.

• Klíčová slova
• Micromass cultures, Mineralised matrix, Mouse limbs, Osteogenesis, PCR Array,
• MeSH
• buněčná diferenciace fyziologie   MeSH
• chondrogeneze fyziologie   MeSH
• kosti a kostní tkáň metabolismus   MeSH
• myši MeSH
• osteoblasty cytologie   metabolismus   MeSH
• osteogeneze fyziologie   MeSH
• osteokalcin metabolismus   MeSH
• protoonkogenní proteiny c-myb genetika   metabolismus   MeSH
• upregulace MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• osteokalcin MeSH
• protoonkogenní proteiny c-myb MeSH