The serine proteinase (SP) released into the environment by most strains of S. aureus cleaves human IgG, IgM and IgA of both subclasses--IgA 1 and IgA 2. SP cleaves H chains of all immunoglobulin classes and the SC of S-IgA, the L chains are degraded partially. The SP-induced cleavage results in a large spectrum of fragments under reducing conditions within a broad range of Mr (approx. 41,000 to less than 12,400). This indicates that the enzyme does not affect the Ig molecule in the hinge region only. The degree of cleavage depends on the enzyme:substrate ratio and on the duration of incubation. The generation of small fragments is associated with the loss of antigenic determinants that results from the decreased binding of the cleaved material in the ELISA method. Partial cleavage of L chains suggests that the enzyme alters part of the molecule that is involved in antigen binding. Even if the ability of antigen binding remains preserved after cleaving Ig with SP, the antibody function is disturbed by splitting off the Fc region or by its degradation into small fragments. SP has to be considered as one of the virulence factors of S. aureus that may protect bacteria against the defence mechanisms of the host.

• MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• ELISA MeSH
• epitopy metabolismus   MeSH
• imunoglobulin A sekreční chemie   metabolismus   MeSH
• imunoglobulin G chemie   metabolismus   MeSH
• imunoglobulin M chemie   metabolismus   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• peptidové fragmenty MeSH
• serinové endopeptidasy chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• epitopy MeSH
• glutamyl endopeptidase MeSH Prohlížeč
• imunoglobulin A sekreční MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• peptidové fragmenty MeSH
• serinové endopeptidasy MeSH

• MeSH
• bakteriální proteiny metabolismus   MeSH
• cysteinové endopeptidasy metabolismus   MeSH
• imunoglobulin A sekreční metabolismus   MeSH
• imunoglobulin A metabolismus   MeSH
• imunoglobulin G metabolismus   MeSH
• imunoglobulin M metabolismus   MeSH
• kolostrum imunologie   MeSH
• lidé MeSH
• metaloendopeptidasy metabolismus   MeSH
• myelomové proteiny metabolismus   MeSH
• serinové endopeptidasy metabolismus   MeSH
• Staphylococcus aureus enzymologie   MeSH
• substrátová specifita MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• bakteriální proteiny MeSH
• cysteinové endopeptidasy MeSH
• IgA-specific serine endopeptidase MeSH Prohlížeč
• imunoglobulin A sekreční MeSH
• imunoglobulin A MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• metaloendopeptidasy MeSH
• myelomové proteiny MeSH
• pseudolysin, Pseudomonas aeruginosa MeSH Prohlížeč
• serinové endopeptidasy MeSH