beta-Tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. Post-translational modifications of brain non-class-III beta-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. Less extensive modifications were also observed in Neuro-2a, HeLa and 3T3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. The data suggest post-translational modification of non-class-III beta-tubulin isoforms in neuronal as well as in non-neuronal cells. Such modification might modulate interaction of tubulin with microtubule-associated proteins.

• MeSH
• 3T3 Cells MeSH
• Species Specificity MeSH
• Electrophoresis, Polyacrylamide Gel MeSH
• Fibroblasts metabolism   MeSH
• HeLa Cells MeSH
• Cells, Cultured MeSH
• Humans MeSH
• Brain metabolism   MeSH
• Mice MeSH
• Neuroblastoma MeSH
• Neurons metabolism   MeSH
• Protein Processing, Post-Translational MeSH
• Tubulin metabolism   MeSH
• Blotting, Western MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Tubulin MeSH