EDTA, ethylenediaminetetraacetic acid Dotaz Zobrazit nápovědu

198 záznamů v PubMed

Článek

Simultaneous capillary electrophoretic separation of small anions and cations after complexation with ethylenediaminetetraacetic acid

Metal cations in the sample are transformed into their chelates with EDTA and are separated together ...

Journal of chromatography. A. 1999 Mar 19 ; 836 (1) : 75-80.

J Chromatogr A
ISSN 0021-9673
Zdroj

A new approach for simultaneous separation of small inorganic and organic anions and metal cations by capillary electrophoresis is demonstrated. Metal cations in the sample are transformed into their chelates with EDTA and are separated together with the anions using an anionic separation mode. Simultaneous separation of 19 common anions and cations was achieved in about 6 min with the electrolyte containing 5 mM K2CrO4, 3 mM boric acid, 35 microM cetyltrimethylammonium bromide and 12 microM EDTA at pH 8. Limits of detection (s/n = 3) were in the range from 4 ppb for Cl- up to 1250 ppb for Cu-EDTA and RSDs of peak areas ranged from 1.4% for Cl- up to 8.5% for Mn-EDTA chelate. The practical applicability of the method was demonstrated on the analysis of anions and cations in various water samples.

Článek

Diagnostický význam komplexonu (CaNa2 EDTA) u saturnismu
[Diagnostic importance of complexon (CaNa2, EDTA) in lead poisoning]

Pracovni lekarstvi. 1957 Apr ; 9 (2) : 134-7.

Prac Lek
ISSN 0032-6291
Zdroj

Klíčová slova
EDATHAMIL *, LEAD POISONING/diagnosis *,
MeSH
chelátory * MeSH
EDTA * MeSH
lidé MeSH
otrava olovem diagnóza MeSH
Check Tag
lidé MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
chelátory * MeSH
EDTA * MeSH

Článek

Stealthy Player in Lipid Experiments? EDTA Binding to Phosphatidylcholine Membranes Probed by Simulations and Monolayer Experiments

Ethylenediaminetetraacetic acid (EDTA) is frequently used in lipid experiments to remove redundant ions ...

The journal of physical chemistry. B. 2023 Jun 22 ; 127 (24) : 5462-5469. [epub] 20230612

J Phys Chem B
ISSN 1520-5207
Zdroj

Ethylenediaminetetraacetic acid (EDTA) is frequently used in lipid experiments to remove redundant ions, such as Ca2+, from the sample solution. In this work, combining molecular dynamics (MD) simulations and Langmuir monolayer experiments, we show that on top of the expected Ca2+ depletion, EDTA anions themselves bind to phosphatidylcholine (PC) monolayers. This binding, originating from EDTA interaction with choline groups of PC lipids, leads to the adsorption of EDTA anions at the monolayer surface and concentration-dependent changes in surface pressure as measured by monolayer experiments and explained by MD simulations. This surprising observation emphasizes that lipid experiments carried out using EDTA-containing solutions, especially of high concentrations, must be interpreted very carefully due to potential interfering interactions of EDTA with lipids and other biomolecules involved in the experiment, e.g., cationic peptides, that may alter membrane-binding affinities of studied compounds.