The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.

• MeSH
• fluorescenční protilátková technika * MeSH
• fosfopyruváthydratasa analýza   imunologie   MeSH
• kmenové buňky chemie   cytologie   MeSH
• kultivované buňky MeSH
• lidé MeSH
• limbus corneae cytologie   MeSH
• monoklonální protilátky MeSH
• transplantace rohovky * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• fosfopyruváthydratasa MeSH
• monoklonální protilátky MeSH