Repeated cultivations (4 passages) of salmonellae (18 strains) resistant to cadmium, streptomycin and beta-lactam antibiotics in Müller-Hinton Broth (MHB) and Mac-Conkey Broth (MCB) without and with CdSO4 (2, 20 and 100 mg/L) showed a higher toxic effect of cadmium in MCB. The strains survived at CdSO4 100 mg/L in MHB for four transfers, in MCB only a single transfer. In dependence on the medium used and amount of metal added, the increase of resistance to antibiotics was different. In MHB, the same levels of resistance to carbenicillin and streptomycin were induced by CdSO4 (20 and 100 mg/L), in MCB it was by 2 and 20 mg/L. Simultaneous stop of the growth of a control culture S. typhimurium with chromosomal resistance to streptomycin, isolates with and without plasmid in MCB which contained CdSO4 100 mg/L, and the results of conjugal transfer of resistance suggest that changes of resistance to antibiotics were not mediated by determinants of resistance to antibiotics. The binding of cadmium to outer membrane protein can cause a decreased permeability to these antibiotics as a resistance mechanism.
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Microbial * genetics MeSH
- Escherichia coli drug effects genetics MeSH
- Cadmium pharmacology MeSH
- Conjugation, Genetic MeSH
- Lactams MeSH
- Drug Resistance, Multiple genetics MeSH
- Swine MeSH
- R Factors genetics MeSH
- Salmonella drug effects genetics isolation & purification MeSH
- Streptomycin pharmacology MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Cadmium MeSH
- Lactams MeSH
- Streptomycin MeSH
